Abstract
A new, simple, rapid, wide applicable range and reliable second derivative spectrophotometric method has been developed for determination of cyclophosphamide (CP) in bulk and pharmaceutical dosage forms. Calibration graph is linear in the concentration range of 25 - 200 μg/ml of CP with 10 μg/ml of detection limit and correlation coefficient of 0.9976. The precision and accuracy were acceptable depending upon the values of relative standard deviation and error percentage. Developed second derivative spectrophotometric method can be directly and easily applied for analyzing pure form and commercial pharmaceutical preparations of CP. The method was compared with a standard high performance liquid chromatography (HPLC) method and can be used for the quality control of pharmaceutical preparations in Erbil City. Key words : Cyclophosphamide, derivative spectrophotometry.
Highlights
Cyclophosphamide (CP) is widely used in cancer chemotherapy, mostly in combination with other antineoplastic agents, and as an immunosuppressant (Karahalil and Akkoyunlu, 2003)
The method was compared with a standard high performance liquid chromatography (HPLC) method and can be used for the quality control of pharmaceutical preparations in Erbil City
The aim of this study was to develop a simple, rapid and efficient second derivative spectrophotometric method for the determination of CP in pharmaceutical formulations and compared with HPLC method that can be used for the quality control of pharmaceutical preparations in Erbil City
Summary
Cyclophosphamide (CP) is widely used in cancer chemotherapy, mostly in combination with other antineoplastic agents, and as an immunosuppressant (Karahalil and Akkoyunlu, 2003). Several analytical methods have been reported and published for quantitative determination of CP in biological fluid and environmental samples, such as: High performance liquid chromatography (HPLC) methods (Malothu et al, 2009; Ahmad et al, 2011), gas chromatography (GC)-mass spectrometry (Sugiura et al, 2010), and after extraction of CP by solid phase extraction techniques, using solid-phase extraction and GC-MS spectrometry (Martins et al, 2004), on-line sample preparation method by micro-extraction packed sorbent (MEPS) followed by LCMS/MS (Kamel et al, 2009), liquid chromatography with diode array detector (Alcântara et al, 2010), and spectrophotometric method (Karen et al, 2009). There are no derivative spectrophotometric methods concerning the determination of cyclophosphamide in pharmaceutical formulations.
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