Abstract
CMCase production by immobilized Aspergillus niger in submerged fermentation was studied .The isolated culture of Aspergillus niger was immob ilized in calcium alginate beads. Studies were carried out on different parameters li ke alginate concentration, incubation time, bead inoculum, CaCl2 concentration and curing time, which affect the pr oductivity and stability of the immobilized system. The maximum CM Case activity of 0.37 IU/ml was achieved with 4% alginate concentration, 48h of inc ubation time, 40 beads/flask of inoculum, 0.2M CaCl2 concentration and 60 min of curing time.
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