Abstract
The management of colored cotton is an agricultural activity widely adopted by farmers located at Brazilian semiarid region. The fiber colors currently available are still limited to green and shades of brown, however, there is possibility to broaden the variability for this trait by using accessions from Gossypium Brazilian bank in breeding programs. Therefore, it is necessary to know the genetic diversity of available accessions in the collection. Here, the genetic divergence in colored fiber accessions was estimated in order to identify promising candidates for further use in hybridization procedures of cotton improvement. DNA of twelve accessions were extracted from leaves and used in inter simple sequence repeat-polymerase chain reaction (ISSR-PCR) assays, using commercial oligonucleotides. The genetic divergence was estimated by clustering-unweighted pair group method with arithmetic mean (UPGMA) method. Five groups were clustered among them, three were contributive results for further use in hybridization procedures, including Brazilian cultivars and Peruvian accessions. Based on level of divergence, we suggest that lines generated from these materials could generate news shades of fiber colors in further use for selection procedures in cotton breeding. Key words: Gossypium, molecular marker, variability, genetic improvement.
Highlights
The fiber colors currently available are still limited to green and shades of brown, there is possibility to broaden the variability for this trait by using accessions from Gossypium Brazilian bank in breeding programs
Based on level of divergence, we suggest that lines generated from these materials could generate news shades of fiber colors in further use for selection procedures in cotton breeding
In this work the genetic divergence of new lines of cultivated and wild Gossypium accessions were estimated based on polymerase chain reaction-inter simple sequence repeat (PCR-ISSR) molecular markers
Summary
Plant genetic resources represent a valorous portion of the biological diversity and contribute towards achieving security and sustainable development from preservation of cultivars, landraces, and wild relatives of important. The Brazilian collection is maintained by the Brazilian Agricultural Research Corporation (Embrapa) at the National Center for Genetic Resources and Biotechnology and currently has more than 3,000 accessions, several of them used for cotton breeding program to Savanna (Cerrado) and semiarid regions. Other niches have emerged, such as the naturally colored fibers, that required no or less dying in the textile processing, reducing the pollution to the environment due to minor residual chemical toxicant (Xiao et al, 2007; Yuan et al, 2012; Feng et al, 2013) This technology is a differentiated product and with higher valueadded, representing an alternative model of innovation, to promote social and sustainable transformations (Cavalcanti, 2012). In this work the genetic divergence of new lines of cultivated and wild Gossypium accessions were estimated based on polymerase chain reaction-inter simple sequence repeat (PCR-ISSR) molecular markers
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