English

C Anyika Kingsley,A Rayyanu Usman,Igomu Elayoni,I Ogbu Kenneth,S Bata Ishaku,I Ankeli Paul,T Ikpa Livinus,P Mayowa Olabode,D Luka Pam

doi:10.5897/ajmr2021.9605

Abstract

Control of contagious bovine pleuropneumonia (CBPP) is difficult in Nigeria due to lack of sufficient data. Mycoplasma mycoides subsp. mycoides is the causative organism of CBPP. This disease is endemic in Nigeria with serious economic impact. This study was undertaken to characterize M. mycoides subsp. mycoides (Mmm) in cattle in south-east Nigeria using single-locus sequence analysis based on polymorphism analysis of non-coding genes and also comparison of the obtained sequences with the vaccine reference strain (T1/44) that is presently used in Nigeria. Twenty one Polymerase chain reaction (PCR) confirmed Mmm isolates from Anambra and Enugu states of Nigeria were used for this study. The amplicons were amplified using one locus of the PG1 reference strain (LocPG1-0001) and sequenced using the corresponding primers. The sequences were edited using BioEdittm software and a consensus sequence obtained. Thereafter, the consensus sequence was aligned using ClustalW (BioEdittm). The consensus sequences obtained were compared with the PG1 reference strain and allele numbers were assigned based on any nucleotide changes. Three allelic numbers were obtained; Allele 1, 8 and 9.  Allele number 8 and 9 are new findings as they were not previously reported in Nigeria. Points of polymorphism were observed between the vaccine strain and the field isolates. The 21 sequences were deposited in the Genbank with the following accession numbers; MW487814-MW487834.     Key words: Alleles, multi-locus sequence analysis, Mycoplasma mycoides subsp. mycoides, Nigeria, polymorphism.

Highlights

Mycoplasma mycoides subsp. mycoides (Mmm) is the causative organism of contagious bovine pleuropneumonia (CBPP) (Thiaucourt et al, 2006)
Twenty one of the Polymerase chain reaction (PCR) confirmed M. mycoides subsp mycoides isolates were of the expected band size of the selected locus, Loc-PG1-0001 as indicated by the production of a band size equivalent to 538 bp (Figure 2)
Contagious bovine pleuropneumonia is a trans-boundary animal disease, with economic effects estimated at billions of Naira in Nigeria (Fadiga et al, 2013)

Summary

Introduction

Mycoplasma mycoides subsp. mycoides (Mmm) is the causative organism of contagious bovine pleuropneumonia (CBPP) (Thiaucourt et al, 2006). Mycoides (Mmm) is the causative organism of contagious bovine pleuropneumonia (CBPP) (Thiaucourt et al, 2006). It is a highly contagious and fatal disease of cattle, causing. Many pastoralists have adopted ‘the live with the disease’ perspective in Nigeria (Chima et al, 2001) This has resulted in the heavy usage of antibiotics for the treatment of the disease and reporting it (Chima et al, 2001). Lorenzon et al (2003), identified 15 different allelic profiles of Mmm from 48 different strains using a technique that involves sequencing multiple loci, termed Multi Locus Sequence Analysis (MLSA). It is a very robust approach (Yaya et al, 2008)

Methods

Results

Conclusion