Abstract
A protease producing microorganism was isolated from soil collected from a detergent industry and identified as Bacillus species. Isolate K-30 produced thermostable alkaline protease utilizing rice bran. The optimum conditions for protease activity was 55°C at pH 9 with 4% inoculum in the medium containing 1% rice bran after 96 h of incubation. Beef extract, tryptone and yeast extract were good nitrogen sources while lactose, starch, and sucrose were suitable for enzyme production. The extracellular production of the enzyme, its thermostable nature and compatibility with most commercial detergents are features which suggest its application in the detergent industry.
Highlights
Proteases are essential constituents of all forms of life on earth including prokaryotes, fungi, plants and animals
A protease producing microorganism was isolated from soil collected from a detergent industry and identified as Bacillus species
Alkaline proteases produced by Bacillus species are of great importance in detergent industry due to their high thermostability and pH stability
Summary
A protease producing microorganism was isolated from soil collected from a detergent industry and identified as Bacillus species. Isolate K-30 produced thermostable alkaline protease utilizing rice bran. The optimum conditions for protease activity was 55°C at pH 9 with 4% inoculum in the medium containing 1% rice bran after 96 h of incubation. Tryptone and yeast extract were good nitrogen sources while lactose, starch, and sucrose were suitable for enzyme production. The extracellular production of the enzyme, its thermostable nature and compatibility with most commercial detergents are features which suggest its application in the detergent industry
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
