Abstract
RNA interference is a post- transcriptional gene regulation mechanism that is predominantly found in eukaryotic organisms. RNAi demonstrated a successful gene silencing in insects that led to the development of novel approaches for insect pest management. In the current study, genes encoding vacuolar ATPase (V-ATPase) subunits a and c from the midgut of pink bollworm, Pectinophora gossypiella, were cloned and sequenced. The full length of V-ATPase subunits a and c cDNAs are 2526 and 1140 bp, respectively. The silencing effect of RNAi on these two genes was determined by microinjecting three dsRNA fragments into the thoracic region of pink bollworm larvae. Bioassay results revealed that 200 ng of dsRNAs silenced both genes causing mortality of 18.9 to 26.7%. Key words: RNAi, dsRNA, Pink bollworm, vacuolar ATPase subunit a, vacuolar ATPase subunit c.
Highlights
RNA interference (RNAi) is a mechanism of posttranscriptional regulation in higher eukaryotes inhibiting gene expression by RNA transcript degradation (Berezikov, 2011)
RNAi demonstrated a successful gene silencing in insects that led to the development of novel approaches for insect pest management
RNAi technology was harnessed in different applications including insect control, was deployed as a powerful tool in biological control for insect pest management (Gordon and Waterhouse, 2007; Price and Gatehouse, 2008; Zhu, 2013)
Summary
RNA interference (RNAi) is a mechanism of posttranscriptional regulation in higher eukaryotes inhibiting gene expression by RNA transcript degradation (Berezikov, 2011). It was first discovered in the nematode Caenorhabditis elegans by Fire et al (1998) and, later, was exploited as an effective technique for a rapid analysis of gene function by phenotypical changes of the target silenced gene. In this technique, exogenous double stranded RNA (dsRNA) is used to significantly reduce the target gene transcript level (Fire et al, 1998).
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