Abstract
Origanum sipyleum L. (Lamiaceae) is an endemic species of the Western Anatolia, Turkey. Essential oils of oreganos are utilized in pharmaceutical and cosmetic industries. A micropropagation protocol was developed using seedlings-derived explants. Shoot apices of 17d-old seedling were cultured and shoots multiplied on Murashige-Skoog modified (MSM) containing 550 mg/l of CaCl2 for sustained growth. Multiple shoots (3.7 ± 0.3 shoot/explant) were produced on medium containing 1 mg/L benzylaminopurine (BAP). On subculturing rated of shoot multiplication increased to 7.8 ± 0.4. 96% of the shoots rooted in a culture medium with 0.5 mg/L indolebutyric acid (IBA) after 3 weeks. The plantlets were acclimatized into outdoor conditions. 76% of these survived in the greenhouse. These in vitro derived microplants are already under the evaluation for their essential oil composition. Key words: Origanum sipyleum, micropropagation, benzylaminopurine, indolebutyric acid.
Highlights
Oregano (Origanum sp.) is a perennial herb of the family Lamiaceae
We report here on development of tissue culture protocol for propagation using apical tips of this species
A few seeds of O. sipyleum germinated within one week on water-agar
Summary
Origanum sipyleum L. (Lamiaceae) is an endemic species of the Western Anatolia, Turkey. (Lamiaceae) is an endemic species of the Western Anatolia, Turkey. Essential oils of oreganos are utilized in pharmaceutical and cosmetic industries. A micropropagation protocol was developed using seedlings-derived explants. Shoot apices of 17d-old seedling were cultured and shoots multiplied on Murashige-Skoog modified (MSM) containing 550 mg/l of CaCl2 for sustained growth. Multiple shoots (3.7 ± 0.3 shoot/explant) were produced on medium containing 1 mg/L benzylaminopurine (BAP). On subculturing rated of shoot multiplication increased to 7.8 ± 0.4. 96% of the shoots rooted in a culture medium with 0.5 mg/L indolebutyric acid (IBA) after 3 weeks. 76% of these survived in the greenhouse. The plantlets were acclimatized into outdoor conditions. These in vitro derived microplants are already under the evaluation for their essential oil composition
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
