Abstract
The aim of this study was to develop the phylogenetic relationship amongst the ecologically variant Russula species by using polymerase chain reaction (PCR) based technique, random amplified polymorphic DNA (RAPD) and isozyme analyses. Two groups could be characterized amongst the total isolates by cluster analyses. Protease, cellulase, glutamate dehydrogenase, pectinase and acid phosphatase designated band P220.16, C472.18, GLD130.21, Pe569.12 and AP472.12, respectively, were common in all the isolates and four monomorphic RAPD bands viz; 818, 512, 298 and 201 bp were also diversified in the isolates. This common band reveals that diversity of these alleles or loci in all ecologically variant isolates. Thus, the present studies discuss the genetic diversity of ecologically variant Russula species on the basis of RAPD and isozyme analysis.
Highlights
Most of the species of family Rassulaceae are economically important, they inhabit temperate zone and are considered as ectotrophic mycorrhizae associated with forest trees of various families
The present studies discuss the genetic diversity of ecologically variant Russula species on the basis of random amplified polymorphic DNA (RAPD) and isozyme analysis
Some para-morphological criteria especially RAPD and isozyme analysis are used for differentiation of morphologically similar or ecologically variant isolates or both (Micales et al, 1986; Cruickshank and Pitt, 1987; Huhtala et al, 1999; Stanosz et al, 1999; Upadhyay et al, 2003)
Summary
Most of the species of family Rassulaceae are economically important, they inhabit temperate zone and are considered as ectotrophic mycorrhizae associated with forest trees of various families. The common band (enzyme band) reveals that this biochemical (protein) is diversified in all the isolates or in other words the loci or alleles which code to these enzyme bands are diversified in all the isolates (Banke et al, 1997). This concept gives a novel idea for phylogenetic analysis of the Russula species and for other fungi. The basic concept of diversity of alleles or loci in electrogram of RAPD is same as in zymography (Williams et al, 1990; Upadhyay et al, 2003.)
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