Abstract
The effects of Lead Nitrate on catalase (CAT) production levels in post juvenile Clarias gariepinus were investigated. A total of 250 samples of the fish were acclimatized for two week. Six samples of the fish were exposed to the sub-lethal concentrations of 00, 28, 43 and 57 mg/L with replicate in each case for 28 days. Two samples of the fish were randomly selected from the treatments and control for the bioassay of catalase on the 14th and 28th day respectively. The fish organs (kidney, liver and gill) were excised from the fish and homogenized in sodium phosphate buffer (0.2 M, pH 8.0). The data generated were subjected to one-way analysis of variance followed by Duncan Multiple Range test where significant. The results showed that 96 h LC50 of C. gariepinus exposed to lethal concentrations of Lead Nitrate was 284.189 mg/L. The CAT production levels were significantly higher in the kidney of the fish exposed in 28 mg/L on day 14; 43 and 57 mg/L on day 28, respectively. The CAT production levels were significantly higher in the gill of the fish exposed in 57 mg/L on day 14; 43 and 57 mg/L on day 28 respectively. The CAT production levels were significantly higher in the liver of the fish exposed in 28 and 57 mg/L on day 14 respectively. The highest production mean values of 149.55±43.65 and 152.80±40.40 U/mgprotein were obtained in the kidney of the fish exposed in 57 and 43 mg/L, respectively. Therefore, the kidney of the fish exhibited a better control of the toxicant and as such, catalase production level in this organ should be used in assessing the level of physiological changes in the fish. Key words: Catalase, Lead Nitrate, oxidative stress, Clarias gariepinus, LC50.
Highlights
Fish is a rich source of animal protein throughout the world
The catalase production levels in the various organs of C. gariepinus exposed to sublethal concentration of Lead Nitrate varied from concentration to concentration
The CAT production levels were significantly higher in the kidney of the fish exposed in sub-lethal concentration of 28 on day 14; 43 and 57 mg/L on day 28 respectively relative to control
Summary
Fish is a rich source of animal protein throughout the world. Due to its nutritional value (Tingman et al, 2010), the demand for fish food has been on the increase with increasing human population (FAO, 2010, 2012). Fish culture which is an important source of protein and employment for many people (Gabriel et al, 2007) has been used to bridge the gap between demand and supply of fish from capture fisheries. Clarias gariepinus is a member of the Clarridae family. They occur naturally in South East Asia and in Africa and are sometimes called African catfish or mudfish.
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