Abstract
Two potato varieties namely, kufri bahar and kufri surya were tested for in vitro tuberization response to a same culture medium supplemented with three levels of 6-benzly aminopurine (0.75, 1.5 and 2.25 mg/L) in a completely randomized block design. The study was conducted in the Central Potato Research Institute (CPRI), Modipuram, Meerut Campus, and India during the period of 2012 to 2013 and 2013-2014. The objective was to determine optimum concentration of 6-benzly aminopurine for tuberization. Both varieties, exhibited a better response when the culture medium supplemented with 2.25 mg/L of 6-benzyl aminopurine than the other concentrations and control for mean values of number of days for tuber initiation microtuber number, fresh weight of microtubers and number of eyes per microtuber was found optimum and it may be useful to enhance tuber quality as well as crop growth under in vitro conditions at farmer and industrial levels. Key words: In vitro plantlets, potato cultivars, 6- benzyl aminopurine, in vitro plants.
Highlights
In India, potato is an important commercial crop
Two varieties namely kufri bahar and kufri surya were selected for tissue culture studies under lab conditions
In vitro tuberization in potato was studied in kufri bahar and kufri surya varieties
Summary
In India, potato is an important commercial crop (As per the final estimates of 2017-2018, horticulture production stood at record 311.7 MT, which is 3.7% higher than the previous year and 10% higher than the past five years average production area (https://m.economictimes.com/news). India showed tremendous growth in potato production during last one and half decade (Rana and Anwer, 2018). 15% of the total area under potato cultivation around the world is used for the production of tuber seeds (Amina et al, 2006). Production of microtubers is possible throughout the year They have the same health status as in vitro micro plants, and unlike micro plants they do not require hardening (Struik and Wiersema, 1999). The quality in vitro micro plants and micro tubers can be affected by a combination of growth regulators and environmental conditions (Salimi et al, 2010; Yeasmin et al, 2011). Since the effect of BAP on potato micro tuberization has been established, in this experiment, influence of three concentrations (0.75, 1.5 and 2.25 mg/L) of 6-benzyl aminopurine on physical characteristics of microtubers such as microtuber initiation, number of microtubers, weight of microtubers and eyes of microtuber of potato were investigated
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