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Abstract
Powdery mildew disease in chilli pepper caused by Leveillula taurica has been affecting chilli pepper grown in greenhouse and open fields. Inheritance of powdery mildew disease resistance is complex and least understood. In the present study, we identified SCAR markers using F2 mapping populations developed from a cross between a resistant parental line ‘Odisha Local' and a parental line susceptible to powdery mildew ‘9907-9611'. The nucleotide sequence obtained from a genome of resistant chilli pepper line ‘Odisha Local’ using OPA15 primer showed partial identity with RPP13 like disease resistant protein. The molecular markers developed in this study will be very helpful in chilli pepper breeding programs for powdery mildew resistance for indirect selection of the resistant plants. Key words: Chilli pepper, powdery mildew, SCAR markers.
Highlights
Chilli pepper (Capsicum annuum L.) is an economically important spice crop that is widely cultivated in India as well as in tropical and sub-tropical countries
We identified sequencecharacterized amplified regions (SCARs) markers using F2 mapping populations developed from a cross between a resistant parental line ‘Odisha Local' and a parental line susceptible to powdery mildew ‘9907-9611'
In rainy season of 2013, we evaluated 199 F2 plant populations along with parental lines for L. taurica disease resistance and observations were taken till 180 days of planting using 0-9 disease scale (Mayee and Datar, 1986) (Table 1)
Summary
Chilli pepper (Capsicum annuum L.) is an economically important spice crop that is widely cultivated in India as well as in tropical and sub-tropical countries. The incidence of the powdery mildew disease in Chilli pepper has been showing an upward trend in both open field and protected net-houses worldwide (Jinkwan et al, 2017). It is one of the important diseases causing up to 80% loss in yield due to severe defoliation and reduction in photosynthesis resulting in less number of fruits and affecting quality of marketable yield (Mathur et al, 1972; Sivaprakasam et al, 1976; Gohokar and Peshney, 1981).
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