Abstract
The microbial community and diversity in an abscisic acid wastewater anaerobic granular sludge system were characterized using molecular techniques. 16S rRNA clone library and sequence analysis revealed that about 99% of 161 bacterial clones belonged to theFirmicutes and Proteobacteria, the remaining 2 clones were assigned to Bacteroidetes.Clostridia (34.8%) and Bacilli (32.3%) belonged to Firmicutes; in the class ofProteobacteria, the clones were assigned to Betaproteobacteria (15.5%),Gammaproteobacteria (13.7%), Alphaproteobacteria (1.2%) and Epsilonproteobacteria(1.2%). For the domain of Archaea, 37.35% of 83 archaeal clones were affiliated withMethanospirillum and Methanocorpusculum respectively; the other of 25.3% belonged toMethanosphaerula. Key words: Abscisic acid wastewater, microbial community and diversity, 16s rRNA, up-flow anaerobic sludge blanket.
Highlights
Industrial wastewater contains organic matters, most of which are poisonous to various life forms and would be a potential hazard to natural water system
The composition and activity of microbial community of anaerobic treatment bioreactor are very important to wipe off organic pollutant of wastewater (Tang et al, 2005; Akarsubasi et al, 2006; Ping et al, 2011), helping us to understand the biotreatment mechanism of waste organic matters, and providing theoretical foundation for large-scale processing
Phylogenetic affiliation of bacterial 16S rRNA gene sequences clones were sequenced and categorized on the basis of their sequence similarity (97% identity). 30 phylotypes were found, among which 13 phylotypes had high levels of similarities with their closest counterparts in public databases, and 17 sequence types showed less than 97% sequence similarity to their nearest database entries (Figure 2)
Summary
Industrial wastewater contains organic matters, most of which are poisonous to various life forms and would be a potential hazard to natural water system. The Up-flow Anaerobic Sludge Blanket (UASB) has been greatly developed, and become one of the most commonly used anaerobic reactors, with great tolerance of organic pollutant load, high efficiency due to short hydraulic retention time (HRT) and low demand of energy (Cintoli et al, 1995; Najafpour et al, 2006). It is widely applied in food, paper, chemical and pharmaceutical industries (Macarie, 2000; Shreeshivadasan and Paul, 2011). The composition and activity of microbial community of anaerobic treatment bioreactor are very important to wipe off organic pollutant of wastewater (Tang et al, 2005; Akarsubasi et al, 2006; Ping et al, 2011), helping us to understand the biotreatment mechanism of waste organic matters, and providing theoretical foundation for large-scale processing
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