Abstract
The effects of sucrose, pH and plant growth hormones on spore germination percentage and gametophyte growths of Pteris tripartita were studied. Various morphological structures of gametophytes were observed namely, filamentous, spatulate and heart stages in the MS culture medium with hormones. After 15 days, the spores of P. tripartita were sprouted in MS basal medium fortified with pH, sucrose and hormones. Maximum spore germination rates (84%) were observed in 70 g/L of sucrose and 79.33% in pH 5.7. On the other hand, the maximum gametophyte sizes were observed both in 40 and 50 g/l of sucrose on half strength MS medium. The maximum growth of gametophyte lengths (484.39 and 507.72 µm) and widths (846.58 and 1270.98 µm) were observed in both pH 5.7 and 6.7. Among three different hormones, the utmost number or percentage of spores were sprouted in GA3. However, the in vitro cultures of spore having the capability to increase the spore germinated due to addition of adequate nutrition in the culture medium and also reduce the contamination as well as environmental factors. Key words: Pteris tripartita, spore germination, sucrose, hormone, MS medium.
Highlights
Pteridophytes have alternation of generations, possessing distinct free-living diploid sporophyte and haploid gametophyte generations
The present investigation was focused on the effects of sucrose, pH and plant growth hormones on spore germination and their gametophyte growths of a critically endangered fern, P
Hundred spores were scored per plate for spore germination percentage and ten gametophytes were randomly selected and measured using microscopes
Summary
Pteridophytes have alternation of generations, possessing distinct free-living diploid sporophyte and haploid gametophyte generations. Fern spore germination and gametophyte development characteristics are much more helpful in taxonomic and phyletic studies (Raine et al, 1996; Chiou and Farrar, 1997; Chiou et al, 1998; Chandra et al., 2003). The present investigation was focused on the effects of sucrose, pH and plant growth hormones on spore germination and their gametophyte growths of a critically endangered fern, P. were randomly selected and observed for the measurement of gametophytes growth. The morphological studies of the gametophytes were measured with the help of stereomicroscope (Nikon SMZ800, Japan) after 45 days of inoculation and photographed using stereomicroscope The micromorphological characters such as meristematic zone, rhizoid formation, perine, obconic cell and shape of protonemal cell were observed. With one way ANOVA test with Duncan’s multiple range test (DMRT) along with p < 0.05 as the limit of significance
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