Abstract
To observe the genetic variability in European honey bee, A. mellifera, PCR was run separately with five primers and analysis of the banding pattern was worked out to investigate the molecular profile of honey bee genotypes collected from different locations having random amplified polymorphic DNA (RAPD) primers. All the five primer screened, amplified the product in between the range of 100 to 1300 bp and 49 scorable markers bands were generated through polymerase chain reaction (PCR), of which 38 (77.55%) were polymorphic and 11 (22.44%) were monomorphic bands identified. Based on the estimated genetic similarity matrix, the highest genetic similarity value (0.861) was noticed between the mid hill region, Jeolikote and tarai region, Pantnagar and lowest genetic similarity value (0.375) was observed between Haldwani and Rajasthan. The major gene cluster consisted of eight European honey bee, A. mellifera accessions from Haldwani, Almora, Do-gaun, Jeolikote, Fatehpur, Ramnagar, Aligarh, Pantnagar, while the minor gene cluster comprised single accession from Rajasthan.
Highlights
European honeybee (Apis mellifera L.) has been object of numerous studies and extensively used from different points of view like genetic, morphometrical and molecular studies (Ruttner, 1988)
The major gene cluster consisted of eight European honey bee, A. mellifera accessions from Haldwani, Almora, Do-gaun, Jeolikote, Fatehpur, Ramnagar, Aligarh, Pantnagar, while the minor gene cluster comprised single accession from Rajasthan
The results obtained from the present investigation are presented under the following subheads: DNA fingerprinting of European honey bee, Apis mellifera L
Summary
European honeybee (Apis mellifera L.) has been object of numerous studies and extensively used from different points of view like genetic, morphometrical and molecular studies (Ruttner, 1988). The need to conserve the genetic diversity of domesticated plants is well docu-mented (Rogers, 2004). Scherf (2000) drew attention to the loss of genetic diversity in livestock worldwide, focusing on domesticated mammals and birds. Despite the widespread consensus that preserving the genetic diversity of domesticated species may prove valuable to humanity, there have been few efforts to preserve the genetic diversity of beneficial arthropod species. While several arthropod species are cultured by humans, A. mellifera L., the western honey bee, is the most economically important beneficial insect. Insects comprises the largest species composition in the entire animal kingdom and posses a vast undiscovered genetic diversity and gene pool that can be better explored using molecular marker techniques. Even within a species varies, in their behavior and morphology that attributes to their complex interaction with the environment (Dempster and McLean, 1999)
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