Abstract
Plant growth-promoting rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and enhance plant growth by a wide variety of mechanisms. The use of PGPR is steadily increasing in agriculture and offers an attractive way to replace chemical fertilizers, pesticides and supplements. In the present study, isolation and characterization of PGPR from the rhizospheric soil of wheat was done for the enhancement of wheat growth. Rhizospheric soils samples were collected from different areas of Uttar Pradesh, India. Twenty two (22) isolates of bacteria were successfully isolated, biochemically characterized and screened for their plant growth promoting traits like production of indole acetic acid (IAA), ammonia production, siderophore production and phosphate solubilization. On the basis of multiple plant growth promoting activities among 22 isolates, 17 isolates were also evaluated for their quantitative production of IAA. The isolates Ps-AB4, Ps-BK1 and Ps-JN 2 showed highest IAA production (79.67-110.12 µg/L). Out of 22, 20 and 6 isolates produced ammonia and siderophore, respectively, while 10 isolates solubilized phosphate on the Pikovskaya’s agar medium. The present study reveals that the use of Pseudomonas isolates Ps-AB4, Ps-BK1 and Ps-JN2 as inoculants biofertilizers might be beneficial for wheat cultivation. Key words: Indole acetic acid, NH3, Pseudomonas spp., phosphate solubilization, wheat.
Highlights
Bacteria that colonize the rhizosphere and plant roots, and enhance plant growth by any mechanism are referred to as plant growth-promoting rhizobacteria (PGPR)
In addition to improvement of plant growth, PGPR are directly involved in increased uptake of nitrogen, synthesis of phytohormones, solubilization of minerals such as phosphorus, and production of siderophore that chelate iron and make it available to the plant root (Mohite, 2013; Schoebitz et al, 2013)
Wheat is the most important staple food in several developing countries, and chemical fertilizer is the most important input required for wheat cultivation
Summary
Bacteria that colonize the rhizosphere and plant roots, and enhance plant growth by any mechanism are referred to as plant growth-promoting rhizobacteria (PGPR). All the bacterial isolates streaked on the surface of Pikovskaya’s agar plate and phosphate solubilizing activity was estimated after 4 days of incubation at 28°C. Round colonies to entire margin grown culture were inoculated in 10 mL peptone water in each test tube and incubated for four days at 30°C.
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