Abstract
Thirty-two isolates of Fusarium oxysporum f. sp. lentis were isolated from wilted lentil plants collected from different lentil growing areas in north-west Algeria. A pathogenicity test was performed for all isolates. Results indicated that the Fol isolates represent a single race but differ in their aggressiveness on the susceptible lines. The amount of genetic variation was evaluated by polymerase chain reaction (PCR) amplification with a set of 6 RAPD primers and 3 AFLP selective nucleotide primer pairs. All amplifications revealed scorable polymorphisms among the isolates, and a total of 8 polymorphic fragments were scored for the RAPD primers and 93 for the AFLP primers. Genetic similarity between each of the isolates was calculated by using the Jaccard similarity coefficient and cluster analysis was used to generate a dendrogram showing relationship between them. The isolates could be grouped into two subpopulations based on RAPD and AFLP analysis. Results obtained indicate that there is little genetic variability among a subpopulation of Fol as identified by RAPD and AFLP markers and that there is no apparent correlation with geographical origin or aggressiveness of isolates. Also, the data suggest that Fol isolates are derived from two genetically distinct clonal lineages. Key Words: Lens culinaris , Fusarium wilt, molecular markers, pathogenicity. African Journal of Biotechnology Vol.3(1) 2004: 25-31
Highlights
Lentil (Lens culinaris Med.) wilt caused by Fusarium oxysporum Schlecht. emend
The objective of this study was to determine the utility of two rapid polymerase chain reaction (PCR)-based approaches, Random Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) markers to determine the genetic structure of a collection of isolates obtained from diseased lentils in Algeria, their relation to pathogenicity, considering that genetic variation could indicate variability of the local population of Fol
The pathogenicity study showed that the behaviour of Fol isolates was homogeneous, with no variations in virulence
Summary
Lentil (Lens culinaris Med.) wilt caused by Fusarium oxysporum Schlecht. emend. Snyder & Hansen f. sp. lentis Vasudeva and Srinivasan (Fol), is the most important disease affecting lentils in Algeria, causing significant economic losses (Setti and Bouznad, 1998; Belabid et al, 2000). Lentis Vasudeva and Srinivasan (Fol), is the most important disease affecting lentils in Algeria, causing significant economic losses (Setti and Bouznad, 1998; Belabid et al, 2000). The pathogen persists in the soil as chlamydospores that can remain viable for several seasons (Erskine and Bayaa, 1996), and is capable of colonizing crop residue and roots of most crops grown in rotation with lentil. It can enter the host through root tips, primarily in the area of elongation, a process which is aided by wounding (Bhalla et al, 1992).
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