Abstract
The experimental material consisted of two parents Gurjari (white backed plant hopper resistant) and Jaya (white backed plant hopper susceptible) and their F2 progeny. The purpose of the study was the identification of RAPD (Random Amplified Polymorphic DNA) marker for white backed plant hopper (WBPH) resistant gene. The RAPD analysis was done group wise as well as combined for the bulk segregant analysis (BSA). For the BSA, of the total 50 random primers surveyed, a single linked primer, OPA 08, was identified. This primer generated 8-bands, one of which, OPA08-7, was putatively linked to resistant gene as was evident by its presence in almost all the resistant bulks and vice-versa. This band had molecular weight equal to 1219.38 bp and was found in resistant parent, Gurjari, and in almost all the resistant bulks (the four susceptible bulks revealed absence of the same band) indicating the band OPA08-7 as a marker for WBPH resistance among the screened rice genotypes. Key words: Rice, random amplified polymorphic DNA, white backed plant hopper, resistant, susceptible, gene.
Highlights
The white backed plant hopper (WBPH), Sogetella furcifera (Horvath) (Hemiptera: Delphacidae) is an important pest of rice and has gained a status of serious pest for rice in Asia
The purpose of the study was the identification of RAPD (Random Amplified Polymorphic DNA) marker for white backed plant hopper (WBPH) resistant gene
Out of the total 50 random primers surveyed, a single linked primer, OPA 08 was identified. This primer generated 8-bands, one of which was putatively linked to resistant gene as was evident by its presence in almost all the resistant bulks and vice-versa
Summary
The white backed plant hopper (WBPH), Sogetella furcifera (Horvath) (Hemiptera: Delphacidae) is an important pest of rice and has gained a status of serious pest for rice in Asia. It is widely distributed throughout South and Southeast Asia, India, China, South Pacific Islands and the Northern part of Australia. The insect can completely destroy rice plants, a condition known as hopperburn (Suenaga, 1963). This pest has attained a place of major pest of paddy in Gujarat. Because of the high cost of insecticides and the problem of insecticide-induced WBPH resurgence (Nagata, 1979), varietal resistance is considered as the most promising and practical approach in the integrated control of this pest (Khan and Saxena, 1986)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
