Abstract
The use of fungi for the production of enzymes has been widely investigated, but there are few reports concerning the use of these organisms isolated from the Amazon Region for the production of hydrolytic enzymes. Considering the importance of hydrolases for different industrial applications, this work aimed to select Amazon fungi for the production of cellulase, protease, pectinase, amylase and xylanase. In order to verify the potential of the Amazon isolates for the production of these five hydrolytic enzymes, growth on solid media containing specific substrates was performed. The qualitative assay revealed that among 40 Amazon fungi, 16 produce cellulase; 13 present proteolytic activity; 13 have pectinase activity, 27 produce amylase and 16 present xylanolyitc activity. Two amylase producers were grown in liquid media in order to access their amylolytic activity. Fungi isolated from aloe vera presented higher amylase production within 48 h of cultivation on potato starch broth. Key words: Hydrolytic enzymes, Amazon fungi, solid media assays, amylase activity.
Highlights
Scre eening g of Am mazon fungi for the e produ uction n of hydroly ytic enzzymes s
The use of fungi for the g the use of o these org ganisms isollated from tthe Amazon n Region fo or the produ uction of concerning hydrolytic enzymes
In order to verify the potential p of the Amazon n isolates fo for the prod duction of th hese five hydrolytic enzymes, growth g on solid s media a containing g specific s substrates w was perform med
Summary
Scre eening g of Am mazon fungi for the e produ uction n of hydroly ytic enzzymes s. The use of fungi for the g the use of o these org ganisms isollated from tthe Amazon n Region fo or the produ uction of concerning hydrolytic enzymes. Co onsidering th he importanc ce of hydrola ases for diffe erent industtrial applications, this work aimed d to select Amazon fung gi for the production of cellulase, p protease, pec ctinase, amy ylase and xylanase. In order to verify the potential p of the Amazon n isolates fo for the prod duction of th hese five hydrolytic enzymes, growth g on solid s media a containing g specific s substrates w was perform med.
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