English

Ma Chunyan,Ma Lingbo,Jiang Keji,Song Wei,Zhang Fengying,Ma Hongyu

doi:10.5897/ajb11.973

Abstract

  In this study, we reported 36 novel polymorphic microsatellite markers derived from a cDNA library of Scylla paramamosain. The polymorphisms of these markers were detected in a sample of 24 individuals. A total of 159 alleles were detected with an average of 4.4 alleles per locus. The number of alleles per locus ranged from two to eight. The observed and expected heterozygosity per locus ranged from 0.100 to 1.000 and from 0.097 to 0.819, with an average of 0.679 and 0.625, respectively. Five loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.0014) and no significant linkage disequilibrium between pairs of loci was found. These loci will provide useful information for the studies on genetic diversity and structure, construction of genetic linkage maps and the effectively management of S. paramamosain.   Key words: Scylla paramamosain, microsatellite markers, cDNA, polymorphism.

Highlights

Scylla paramamosain is mainly distributed in the southeastern coastal region of China, as well as in the tropical and warm temperate zones in the Pacific and Indian Oceans (Keenan, 1999)
The aquaculture of S. paramamosain has been carried out for many years in southern China and other Asian countries (Cowan, 1985), but the wildcaught seed was the main source of S. paramamosain farming (Keenan, 1999)
Ease of genotyping and co-dominant inheritance, microsatellite markers are very popular for the study of molecular phylogeography and population genetics (Sun et al, 2009)

Summary

Introduction

Scylla paramamosain is mainly distributed in the southeastern coastal region of China, as well as in the tropical and warm temperate zones in the Pacific and Indian Oceans (Keenan, 1999). Few research based on microsatellite markers are reported for this species. Ease of genotyping and co-dominant inheritance, microsatellite markers are very popular for the study of molecular phylogeography and population genetics (Sun et al, 2009). Isolation of microsatellite markers has been carried out in many important species (Chistiakov et al, 2004; Ma et al, 2009; Ruan et al, 2011).

Results

Conclusion