Abstract
The aim of the present study was to determine the unique molecular markers among, three species of the genus Anthemis and the construction of phylogenetic tree using the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique. Genetic diversity was analyzed among 15 populations of three species of Anthemis (Anthemis melampodina, Anthemis, pseudocotula and Anthemis, bornmuelleri), collected from different locations at Saudi Arabia by using RAPD primers. Pairwise genetic distance was calculated based on Nei and Li coefficient. Unweighted pair group method with an average (UPGMA) was used for construction of dendrogram, based on the similarity matrix data. Results showed wide variations among A. bornmuelleri and other two species. A wide close genetic relation was observed between A. melampodina and A. pseudocotula. RAPD-PCR technique was shown to be an accurate tool, in other to ascertain plant relationships among species of genus Anthemis. Key words: Genetic diversity, random amplified polymorphic DNA-polymerase chain reaction (RAPD- PCR), A. melampodina, A. pseudocotula, A. bornmuelleri.
Highlights
Anthemis L. is one of the largest genus of family Asteraceae, including more than 210 described species (Oberprieler et al, 2007). They are widely distributed across Europe extending into extreme southern Arabia and tropical east Africa and other parts of the world (Oberprieler, 2001)
During the period of March to May 2015, 15 plant samples of three species of Anthemis were collected from different geographic regions in Saudi Arabia through Jizan, Makkah, Hail and Asir (Figure 1, Table 1)
Different approaches in genetic diversity analyses, reveal the different level of polymorphism (Porter and Smith, 1982) and DNA markers are independence of environmental or localities factors which show a greater level of polymorphism (Heywood, 2002)
Summary
Anthemis L. is one of the largest genus of family Asteraceae, including more than 210 described species (Oberprieler et al, 2007). They are widely distributed across Europe extending into extreme southern Arabia and tropical east Africa and other parts of the world (Oberprieler, 2001). Some species inhabit northern America and southern hemisphere as well (Oberprieler, 2001). Some species are widely used in pharmaceutical, cosmetics, and food industry. They are used as herbal tea for treatment of anxiety, flatulence, stomach disorders, insomnia and toothache (Vaverkova et al, 2001)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
