Abstract
Withania somnifera is an important aromatic medicinal plant and possesses wide array of pharmacological properties. In the present investigation, an improved version of hairy root culture system was developed by optimizing various transformation parameters such as type of explant, concentration of acetosyringone, Agrobacterium types and co-cultivation period. Between the leaf and cotyledon explants and two Agrobacterium rhizgenes strains (R1000 and A4) tested, leaf explants infected with R1000 and cocultured for five days on MS basal half strength medium in the presence of acetosyringone (100 µM) attained a higher frequency (88%) of hairy root induction. By adopting this protocol, we could utilize the hairy root culture for industrial scale production of withanolides. Key words: Leaf explant, Agrobacterium rhizogenes, Withania somnifera, co-cultivation period, acetosyringone.
Highlights
An efficien e nt hairy y root culture c e syste em forr Witha ania so omnife era (L.)) Duna al Ganesha an Sivanan ndhan1, Nattesan Selv varaj2, Andy Ganapatthi1 and Markandan
0 and A4) ttested, leaf explants infected witth R1000 and d cocultured d for five day ys on MS ba asal half stre ength medium m in the pre esence of acetosyring gone
In total of 150 leaf explants infected by R1000, an average of 132 leaf explants produced 28.2 number of hairy roots/leaf explant of at least 3 cm in length after 12 days (Table 1)
Summary
An efficien e nt hairy y root culture c e syste em forr Witha ania so omnife era (L.)) Duna al Ganesha an Sivanan ndhan1, Nattesan Selv varaj2, Andy Ganapatthi1 and Markandan. It was observed from the present study that most hairy roots emerged from the wounded sites of midrib of leaf and cotyledon explants infected by both strains used. This study strongly suggests that R1000 was a more effective strain in hairy root induction from 45-day-old leaf explants.
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