Abstract
In the present work, polyploidy was induced in the diploid banana varieties 'Malbut', 'Gold', 'Lidi', and 'Thong Dok Mak' through the use of colchicine and oryzalin, and that condition was identified through stomatal analysis, flow cytometry, and chromosome counts. Shoots produced in vitro were treated with colchicine at concentrations of 0, 2.5, 7.5 and 12.5 mM for 24 and 48 h, and with oryzalin at 0, 10, 30 and 50 mM for 4 and 7 days. Young leaves were scanned by electron microscopy to determine their stomatal areas (polar diameter × equatorial diameter) and numbers for polyploid identification by stomatal analysis. Polyploid identification by way of flow cytometry analysis used samples of young leaves that were crushed to release their nuclei, with subsequent staining with propidium iodide; ten thousand nuclei were analyzed for each sample. For cytogenetic analyses, root tips were pretreated with 0.002 M 8-HQ for 3 h, fixed in Carnoy solution for 24 h, subjected to conventional squashing techniques, and stained with 10% Giemsa. We identified four tetraploid plants and six mixoploids using these three identification techniques. Key words: Chromosomes duplication, Musa acuminata, tissue culture.
Highlights
Le eila Aparec cida Salles s Pio1, Moa acir Pasqual1, Sebasttião de Oliveira e Silv va2, Hermíínio Souza
30.62% when treated with colchicine and 23.12, 15.625, 36.25 and 22.5% when treated with oryzalin respectively
Cytogenetic analyses were only performed on mixoploid or tetraploid plants identified by flow cytometry analyses
Summary
Le eila Aparec cida Salles s Pio, Moa acir Pasqual, Sebasttião de Oliveira e Silv va, Hermíínio Souza. In the prese condition wa 'Thong Dok k Mak' throug gh the use of o colchicine e and oryzaliin, and that c as identified d through stomatal an nalysis, flow cytometry, and a chromos some counts s. Shoots pro oduced in viitro were trea ated with colchicine at a concentra ations of 0, 2.5, 7.5 and 12.5 mM for 2. Young leaves were scanned by electron microscopy y to determine their stomatal arreas (polar diameter × equatorial diameter). D an nd numbers s for polyplo oid identific cation by stomatal an nalysis. Yploid identification by way w of flow cytometry a analysis use ed samples o of young leaves that were crushed to releas se their nuclei, with subs sequent staining with p propidium iod dide; ten thousand nuclei n were analyzed a forr each samp ple. For cyto ogenetic ana alyses, root tips were prretreated with 0.002 M 8-HQ for 3 h, fixed in Carnoy solution s for 24 h, subjected to conventional sq quashing d with 10% Giemsa
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