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Abstract
The microbial communities and diversities in production water from three high-temperature, water-flooded petroleum reservoirs in the same injection area of the Chenghai area of Dagang oil field in China and their recycled injection water sample were characterized by polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) analysis. Results show that microbial sequences mainly belonged to the divisions Proteobacteria, Bacteroidetes, Spirochaetes,Firmicutes and Euryarchaeota. Many mesophilic and thermophilic microorganisms were found in samples of produced water and injection water. Thermoanaerobcter,Desulfotomaculum, Methanolinea, Methanomethylovorans and Geoglobus were detected in all samples. Mesophilic microorganisms found in the samples of produced water, mainly belonged to Brevundimonas, Agrobacterium,Pseudomonas, Sphingomonas, Acinetobacte, Dechloromonas, Clostridium andMethanobacterium. All of them were detected in the injection water samples, exceptBrevundimonas and Methanobacterium, which indicates that injection exploration may be the main reason why mesophilic bacteria was detected in high-temperature petroleum reservoirs of Dagang. Results suggest that reinjection of water not only introduces enormous mesophilic microbe into high-temperature petroleum reservoirs, but also cause thermophilic microorganisms cross-contamination among wells in the same injection area. Key words: Petroleum reservoir, recycled injection water, DGGE, microbial community, cross-contamination.
Highlights
The existence of microorganisms in the subsurface of petroleum reservoir has been known for many years (Bastin et al, 1926), and a great variety of microorganisms have been isolated or detected
All of them were detected in the injection water samples, except Brevundimonas and Methanobacterium, which indicates that injection exploration may be the main reason why mesophilic bacteria was detected in hightemperature petroleum reservoirs of Dagang
This study mainly focuses on analyzing of microbial community and diversity of three production wells and their recycled injection water by ¡£¢¥¤ ¦£§ polymerase chain reaction denaturing gradient gel electrophoresis (PCRDGGE)
Summary
The existence of microorganisms in the subsurface of petroleum reservoir has been known for many years (Bastin et al, 1926), and a great variety of microorganisms have been isolated or detected. Many physiological types of microorganisms have been isolated from different geographic oil reservoirs. These microorganisms include sulfate reducers (Rueter et al, 1994), sulfidogens (Haridon et al, 1995), fermentative bacteria (Grassia et al, 1996), manganese and iron reducers (Greene et al, 1997; Slobodkin et al, 1999), methanogens (Nilsen and Torsvik, 1996), and acetogens (Davydova-Charakhch et al, 1993). Other studies using molecular techniques found that oil reservoirs contain complex microecosystems with thermophilic and mesophilic microbe coexisting (Orphan et al, 2000; Li et al, 2007; Wang et al, 2008; Kaster et al, 2009). The general characteristics of the environment in deep subsurface petroleum reservoirs are high temperature, high salinity, high
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