Abstract
Background: Arabitol dehydrogenase (ArDH) is involved in the production of different sugar alcohols like arabitol, sorbitol, mannitol, erythritol and xylitol by using five carbon sugars as substrate. Arabinose, d-ribose, d-ribulose, xylose and d-xylulose are known substrate of this enzyme. ArDH is mainly produced by osmophilic fungi for the conversion of ribulose to arabitol under stress conditions. Recently this enzyme has been used by various industries for the production of pharmaceutically important sugar alcohols form cheap source than glucose. But the information at structure level as well as its binding energy analysis with different substrates was missing. Results: The present study was focused on sequence analysis, insilico characterization and substrate binding analysis of ArDH from a fungus specie candida albican. Sequence analysis and physicochemical properties showed that this protein is highly stable, negatively charged and having more hydrophilic regions, these properties made this enzyme to bind with number of five carbon sugars as substrate. The predicted 3D model will helpful for further structure based studies. Docking analysis provided free energies of binding of each substrate from a best pose as arabinose -9.8224calK/mol, dribose -11.3701Kcal/mol, d-ribulose -8.9230Kcal/mol, xylose -9.7007Kcal/mol and d-xylulose 9.7802Kcal/mol. Conclusion: Our study provided insight information of structure and interactions of ArDH with its substrate. These results obtained from this study clearly indicate that d-ribose is best substrate for ArDH for the production of sugar alcohols. This information will be helpful for better usage of this enzyme for hyper-production of sugar alcohols by different industries.
Highlights
Arabitol dehydrogenase (ArDH) is involved in the production of different sugar alcohols like arabitol, sorbitol, mannitol, erythritol and xylitol by using five carbon sugars as substrate
Arabitol dehydrogenase is involved in the reduction of a five carbon sugar ribulose to form arabitol in pentose phosphate pathway [1]
This enzyme is produced in various osmophilic fungus species under osmotic stress, to produce compatible solute arabitol to balance the osmotic pressure across the cell membrane
Summary
Arabitol dehydrogenase is involved in the reduction of a five carbon sugar ribulose to form arabitol in pentose phosphate pathway [1]. This enzyme is produced in various osmophilic fungus species under osmotic stress, to produce compatible solute arabitol to balance the osmotic pressure across the cell membrane. The D-arabitol dehydrogenase belongs to the short chain hydrogenases/reductases superfamily It is a group of enzymes with a broad spectrum of substrates and is produced by all domains of life. It catalyzes the oxidation of D-arabitol to D-ribulose and belongs to the class of D-arabitol 2-. The study presents novel insights into the structural features and substrate binding of ArDH
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