Abstract
In recent years, many plants have been used as bioindicators or living indices and several experiments have been conducted to evaluate the genotoxic effects of environmental pollution on plant species. Plants such as Arabidopsis and barley have been used as biological indicators by several researchers and have been able to identify DNA changes using molecular markers. This study was conducted on wheat as an important cereal that provides human daily food and has an important role in human health. The purpose of study was to evaluate the effect of different concentrations of cadmium on amounts of the soluble proteins, investigation of growth and possible changes to the structure of DNA using random amplified polymorphic DNA (RAPD) marker. In the present study, wheat seedlings were used for detection of genotoxic effects of cadmium contamination in the range of 0 to 120 mgl1. The limiting effects of cadmium on length of root and plant height and total soluble proteins in root were quite evident by increase of cadmium concentration. Change in DNA was observed using RAPD marker as there was change in number of bands, present or absent of bands at the range of above 5 mgl1 cadmium concentration. In the previous studies conducted by several researchers, variation in DNA bands was reported at levels above 30 mgl1, whereas in the present study, band variation was observed at the concentration of 5 mgl1. This study also showed that DNA stability is highly affected by cadmium pollution at >5 mgl1 which was identified by RAPD markers. Key words: Bioindicator, cadmium, genotoxic, random amplified polymorphic DNA (RAPD) marker, Triticum aestivum L.
Highlights
In the last several decades, cadmium (Cd) has been used extensively as materials and/or intermediates in the chemical industry and agriculture for electroplating, metallurgy, painting, plastic production, etc., and is being released into the biosphere, and largely found in the water and soil
This study showed that DNA stability is highly affected by cadmium pollution at >5 mgl-1 which was identified by random amplified polymorphic DNA (RAPD) markers
The application of RAPD marker analysis is reported for an assessment of cadmium genotoxicity in wheat
Summary
In the last several decades, cadmium (Cd) has been used extensively as materials and/or intermediates in the chemical industry and agriculture for electroplating, metallurgy, painting, plastic production, etc., and is being released into the biosphere, and largely found in the water and soil The toxic and genotoxic effects of cadmium have been demonstrated in different plant and animal species and it has been shown that cadmium caused change in DNA and cellular structures (Conte et al, 1998; Liu et al, 2005; Shahrtash et al, 2010). RAPD of these techniques can be used to detect genotoxicity and differences in RAPD profiles can clearly be shown when comparing DNA fingerprints from untreated and treated individuals to genotoxic agents (Savva, 1996; Conte et al, 1998; Theodorakis and Bickham, 2001; Enan, 2006; Atienzar and Jha, 2006; Liu et al, 2007; Cenkci et al, 2009). Detection of the genotoxic effect involves comparison of RAPD profiles of the root tip DNA generated by control and treated wheat seedlings
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
