Abstract
The aim of this study was to assess the effect of cooling on sperm motility before and after frozen-thawed stallion semen. Fifteen ejaculates of three stallions were collected with artificial vagina. The progressive motility was determined under microscope immediately after collection, cooling (5°C for 0, 2, 7 or 24 h) before frozen-thawed and cooling (5°C for 0, 2, 7 or 24 h) after the semen was frozen-thawed. Sperm progressive motility (83.1, 78.7, 74.8 or 70.3%, respectively) was significantly different (P<0.05) at different hours of cooling before freezing. Similar pattern was found when semen was subjected to cooling, frozen-thawed and cooling time resulted in a progressive reduction in motility from 39.4 to 26.9%. The motility of semen subjected only to cooling for 24 h before freezing was optimal (70.0%) for artificial insemination. Moreover, semen subjected to cooling for 7 or 24 h before and after frozen-thawed could be used still with some considerations for artificial insemination. Key words: Stallion, semen, motility, cooling, frozen-thawed
Highlights
The best semen quality is obtained when the semen is recently collected by artificial vagina
For different reasons semen must be preserved during different period of times (For example, advanced age or disability to breed by a stallion, to export and/or sell semen, to inseminate estrus synchronized mares that a single stallion may not inseminate or to research, etc)
Preserved semen quality is certainly a factor that impacts the rates of pregnancies in artificial insemination
Summary
The best semen quality is obtained when the semen is recently collected by artificial vagina. Preserved semen quality is certainly a factor that impacts the rates of pregnancies in artificial insemination. A number of studies have been assayed to improve the motility and fertility of stallion semen after cooling and/or frozen-thawed, no satisfactory results have been obtained For the purpose of obtaining satisfactory results, assayed modifications in centrifugations and semen with or without a cushion fluid (Sieme et al, 2006), added substances after cryopreserved spermatozoa (Gradil and Ball, 2000) and removal of seminal plasma (Ramires et al, 2013a; b) had been studied; the incorporation of antioxidant or another.
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