Abstract
An experiment was conducted for induction & establishment of Agrobacterium mediated hairy root culture of Coleus forskohlii was developed & co-cultivation on hormone free semisolid MS Medium with B5vitamins.Sterilization period was standardized for the stem & leaf explant using 0.1% bavistin and 0.1% Hgcl2 with varied intervals It resulted in the emergence of hairy roots from the leaf explant , stem explant after 20th day of infection .The transformation of hairy roots was established and confirmation of forskolin content was done by HPLC. Selection of high forskolin can provide an alternative source of large scale production of forskolin. Keywords- Agrobacterium rhizogene , Coleus forskohlii ,Hairy root, HPLC
Highlights
IntroductionTransformation by Agrobacterium rhizogenes Different strains of Agrobacterium rhizogenes such as A4, ATCC18534, MTCC533 were obtained from different places & standardized a suitable growth medium for A.rhizogene strain Transformation Coleus forskohlii with Ri-plasmid of A. rhizogenes for root organ culture to improve production of secondary metabolites
Coleus forskohlii is a botanical that has been used since ancient times in Hindu and Ayurvedic traditional medicine
Results & Discussion Agrobacterium rhizogene and the specified media were used for transformation work was standardized with Carrot Disk Method
Summary
Transformation by Agrobacterium rhizogenes Different strains of Agrobacterium rhizogenes such as A4, ATCC18534, MTCC533 were obtained from different places & standardized a suitable growth medium for A.rhizogene strain Transformation Coleus forskohlii with Ri-plasmid of A. rhizogenes for root organ culture to improve production of secondary metabolites. The sterile Leaf & stem explants of Coleus forskohlii plants were cut and injured . exised explants used for transformation by Agrobacterium rhizogenes ATCC 15834 was standardized. Five 1-cm tips of roots grown on solid media were placed in 250-ml Erlenmeyer flasks, and well established roots were maintained on Basal M.S media in petriplates and transferred to fermentor when required for large scale production. One-cm tips of roots that grew on the explants were excised and placed on MS medium containing cefotaxime (250mgml21) in petridishes where they grew and branched further.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
