Abstract
The present study focuses on the production of an extracellular alkaline protease enzyme from soil selected strains of various active solid substrates by Bacillus sp., screened from soil. Some fermentation conditions were studied aiming to improve enzyme production-substrate; pH 9.0 and incubation temperature of 37° C given the best results. For the enzyme activity stability, the following best conditions were found: substrate pH of 13.0 and temperature of 55° C. Enzyme production by solid-state fermentation were studied using different agricultural solid wastes like rice bran, wheat bran, coconut oil cake, groundnut oil cake and gingili oil cake. The maximum enzyme activity was achieved from groundnut oil cake with soybean meal as substrate. Key words: Bacillus sp., Solid state fermentation, alkaline protease.
Highlights
Proteases constitute one of the most important groups of industrial enzymes
The present study focuses on the production of an extracellular alkaline protease enzyme from soil selected strains of various active solid substrates by Bacillus sp., screened from soil
Though several fungal sources are being increasingly employed, large properties of the commercially available alkaline proteases are derived from Bacillus sp. (Yang et al, 2000)
Summary
Proteases constitute one of the most important groups of industrial enzymes. They are degradative enzymes, which catalyze the total hydrolysis of protein (Raju et al, 1994). These enzymes are found in a wide diversity of sources such as plants, animals and microorganisms but they are mainly produced by microorganisms like bacteria and fungi. Microbial proteases are the most important industrial enzymes (Chouyyok et al, 2005) that account for approximately 40% of the total worldwide enzymes sale (Godfrey and West, 1996). Alkaline proteases of microbial origin possess considerable industrial potential due to their biochemical diversity and wide applications in tannery and food industries, medicinal formulations, detergents and processes like waste treatment, silver recovery and resolution of amino acid mixtures (Rao et al, 1998). Alkaline proteases were the first enzyme produced in bulk
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