Abstract
The aim of this study was to establish an effective protocol for callus induction from the potato genotypes (Solanum tuberosum L.) used and to investigate whether the transferred oxalate oxidase enzyme or transformation procedure has any effect on callus induction of transgenic lines of cultivar Desiree and Maris Bard. The results showed that the effects of genotype and medium on measured characters were highly significant. In order to compare overall performance of transgenic lines of each cultivar with their parental cultivars (non-transgenic), orthogonal comparisons were used and indicated the significant differences between transgenic and non-transgenic genotypes. The significant differences between the cultivars and their transgenic lines expressing oxalate oxidase enzyme indicated that the transformation procedure had a significant effect on their callus induction and growth. Key words: Callus, Oxalate oxidase, solanum tuberosum, transformation.
Highlights
Much work has been carried out on callus induction and growth in potatoes (Solanum tuberosum L.). This has resulted in a range of protocols and procedures being established by researchers since tissue culture gained an importance in plant propagation, conservation and breeding (Ahloowalia 1982; Wareh et al, 1989)
Previous researches showed that media used for callus induction and growth depends on the genotypes Gonzalez et al (2001, Alexeenko and Irkaeva (1998) pointed out that introduction of genes effecting the structure and type of plant development into strawberry lines influenced callus formation and shoot inducing in vitro
Transformed genotypes expressing oxalate oxidase enzyme were initially conformed by using kanamycin resistance as the selective marker and the oxalate oxsidase enzyme activity as the reporter gene support the expression of transgene
Summary
Much work has been carried out on callus induction and growth in potatoes (Solanum tuberosum L.) This has resulted in a range of protocols and procedures being established by researchers since tissue culture gained an importance in plant propagation, conservation and breeding (Ahloowalia 1982; Wareh et al, 1989). Previous researches showed that media used for callus induction and growth depends on the genotypes Gonzalez et al (2001, Alexeenko and Irkaeva (1998) pointed out that introduction of genes effecting the structure and type of plant development into strawberry lines influenced callus formation and shoot inducing in vitro. Physical disorganization of cultured cells, is thought as result of the breakdown of intercellular physical and chemical communication (Lindsey and Jones, 1992) In this transportation system, vascular bundles in a plant play important roles in terms of transferring water and plant nutrients. Some enzymes such as oxalate oxidase, which is more expressed in salt tolerant species, are thought to have an effect on the vascular system (Hurkman and Tanaka, 1996)
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