Abstract
This study was designed to investigate the ability of ethanol fruit extract from Detarium microcarpum to protect erythrocytes against hemolysis and lipid peroxidation. To achieve this objective, hemolytic, anti-hemolytic and lipid peroxidation from cell membrane assays were used. Hemolytic and anti-hemolytic activities (regarding H2O2 induced hemolysis) were assessed by determination of free haemoglobin at 540 nm. Inhibition of lipid peroxidation was measured at 532 nm, using thiobarbituric acid reaction on sodium nitroprusside and ferric sulfate induced liposome peroxidation models. D. microcarpum fruit ethanol extract (100 µg/ml) did not exhibit any hemolytic effect but reduces significantly hemolysis from human and rat erythrocyte with inhibitory percentages more than 50 and 75%, respectively. Furthermore, the extract caused a significant decrease in both ferric sulfate and sodium nitroprusside inducing lipid peroxidation in each rat tissue liposomes investigated. D. microcarpum fruit ethanol extract protects erythrocytes against hemolysis and lipid peroxidation, probably due to its antioxidant potential. Therefore, animal tissues disorders caused by cell membrane lipid damage could be potentially managed/prevented by dietary intake of D. microcarpum fruit pulp. Key words: Detarium microcarpum, anti-hemolytic activity, inhibition of lipid peroxidation
Highlights
A positive link has been established between fruit consumption and the reduction of chronic diseases (Sumathy et al, 2013)
It was found that D. microcarpum fruit pulp ethanol extract did not affect significantly (p>0.05) the integrity of cell membrane when hemoglobin liberation was compared with control
These results suggest that D. microcarpum fruit pulp ethanol extract (100 μg/mL) did not exhibit a cytotoxic effect on human and rat erythrocytes in vitro
Summary
A positive link has been established between fruit consumption and the reduction of chronic diseases (Sumathy et al, 2013). (Caesalpiniaceae) is a well-known wild edible fruit specie. Its fruits are traditionally consumed as food source and for medicinal purpose (Wahedi et al, 2013). D. microcarpum is used in the treatment of diverse diseases, notably syphilis, dysentery, diarrhea, bronchitis, pneumonia, sore throat, malaria, leprosy and meningitis (Akah et al, 2012) while the fruits are used to treat skin infections, meningitis and malaria (Bamisaye et al, 2014). Fruit pulp extract of D. microcarpum showed an inhibition of the growth of the plant pathogenic fungus, Cladosporium cucumerinum and of the enzyme acetylcholinesterase, implicated in Alzheimer's disease (Cavin et al, 2006)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
