Abstract
The need for alternative strategies to obtain transgenic cotton via apical shoot was necessitated due to the recalcitrance of cotton regeneration from somatic embryogenesis, this has greatly slowed down the development of transgenic cottons. To this effect, an optimized regeneration system from apical shoot was developed for three varieties of cotton. Ninety-five percent seed surface sterility was observed in seed germination using a combination of hydrogen perioxide and Clorox as sterilizing medium. Highest shoot elongation rate was achieved on MS supplemented with 2.5 mg/L BAP + 0.1% (w/v) AC, rapid shoot growth occurred with kinetin supplemented media. Rooting efficiency of the three improved cultivars of cotton (Gossypium hirsutum), Samcot 9,11 and 13 were optimized using the optimum medium for rooting of difficult-to-root in vitro regenerated shoots of cotton which consist of MS basal salts and modified MS vitamins, supplemented with 3% sucrose, 0.2 mg/L IBA, without activated charcoal. In the end, an improved regeneration protocol with rooting efficiency up to 47% and regeneration rate up to 87% by combining rooting induction, indole acetic acid (IAA) shock and graft technique was developed. Key words: Allium sativum, cotton tissue culture, transgenic plant, optimized regeneration of cotton.
Highlights
The focus of research in plant cell culture for many crop species was to be able to put species into tissue culture maintain or grow the plant cells, tissues or organs under sterile controlled laboratory conditions and regenerate a normal fertile plant
The tiny seedlings were transferred to different media supplemented with plant growth regulators (BAP, NAA, IBA) and activated charcoal (AC)
The highest rate of elongation was achieved on MS supplemented with 2.5 mg/L BAP +
Summary
The focus of research in plant cell culture for many crop species was to be able to put species into tissue culture maintain or grow the plant cells, tissues or organs under sterile controlled laboratory conditions and regenerate a normal fertile plant. In vitro cultured cotton cells have been induced to undergo somatic embryogenesis in numerous laboratories using varied strategies (Shoemaker et al, 1986; Chen et al, 1987; Trolinder and Goodin, 1987; Kolganova et al, 1992; Zhang, 1994a; Zhang et al, 1996, 1999). Regenerated plants have been obtained from explants such as hypocotyls, cotyledon, root (Zhang, 1994a) and from various cotton species (Zhang, 1994b).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
