Abstract
Bifidobacteria have been in the spotlight of scientific research due to their health promoting effects in humans. They play a significant role in controlling pH of the large intestine through liberation of lactic and acetic acid, thereby restricting the growth of many potential pathogens and putrefactive bacteria. This paper discusses the isolation and identification of bifidobacterial species from infant faeces by molecular methods. A study was conducted to isolate and identify bifidobacterial species from the faeces of 46 breast fed infants. Molecular tools like 16S rDNA targeted genus and species specific mPCR primers were used for confirmation. A total of 4 isolates were confirmed namely Bifidobacterium longum (Isolate code: IB10 and IB12) Bifidobacterium breve (Isolate code: IB39) and Bifidobacterium bifidum (Isolate code: IB42). Subsequently, the nucleotide sequences of the identified species were submitted to the GenBank for acquisition of accession numbers. The accession numbers assigned to the isolates were JN 656706 and JN 656707 for B. longum (IB10) and B. longum (IB12), respectively; JN 656708 for B. breve (IB39) and JN 656705 for B. bifidum (IB42). Key words: Faecal Bifidobacteria, multiplex polymerase chain reaction (PCR) for Bifidobacteria, 16S rDNABifdobacteria, isolation of Bifidobacteria.
Highlights
Recent research has led to rapid advances in the application of molecular techniques based on 16S rDNA and 23S rDNA
Multiplex PCR was done with primers for B. adolescentis, B. bifidum, B. breve, B. longum and B
Giovanna and Dellaglio (2007) studied the phylogenetic tree based on 16S rDNA gene sequence analysis showing the phylogenetic relationships among the species of Bifidobacterium
Summary
29 bifidobacterial species have been identified and a among them elleven species have been n isolated fro om infa ant faeces Th he most freque ently isolated Bifidobacteriu um e Bifidobacte erium bifidum m, speciies in infantt faeces are obacterium llongum, Bifid dobacterium infantis and d. Bifido obacterium brreve (Matsukii et al, 2003) These Gram m positiive, non motile, non sporre forming an naerobic pleo omorp hic rods playy a significantt role as prob biotics. These e nisms controll the pH of tthe large inte estine through h organ produ uction of lacticc and acetic acid thereby restricting the e growtth of many potential pa athogens and d putrefactive e bacte eria
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