Abstract
The enzyme invertase is very useful, especially in food industries. Invertase hydrolyzes the disaccharide sucrose into two monosaccharides, glucose and fructose. Invertase was extracted from Aspergillus sp. CSA35, and studied using spectrophotometric methods for enzyme kinetics and determination of other factors affecting enzyme activities such as salt tolerance, pH, temperature and substrate specificity. The invertase secreted by Aspergillus sp. CSA35 has a substrate concentration (km) value of 1.25 mg/ml and maximum velocity (Vmax) of 15.15 U/mg protein. It is tolerant to salt concentrations of 3 M NaCl for 24 h and shows a broad range of substrate specificity. The enzyme activity was optimal at pH 6.0 and at a temperature of 50°C. The Vmax of the enzyme was high compared to other invertases from fungal sources, previously reported in literature. This invertase could be useful in commercial processes where high activity invertase with salt-tolerant and broad specific properties are required. Key words: Aspergillus sp CSA35, invertase, Manihot esculenta.
Highlights
Enzymes are biomolecules synthesized by the cells of an organism to allow biochemical reactions to occur under mild conditions and are known to catalyse many biochemical reactions (Li et al, 2018)
This study was aimed at producing invertase enzyme from Aspergillus CSA35 isolate and to evaluate parameters such as salt tolerance, kinetic properties, pH, temperature and substrate specificity in order to determine optimum conditions in which this crude invertase extracted from Aspergillus sp.CSA35 would maximally express its catalytic activity
As calculated from the Lineweaver–Burk plots (Figure 2), the Vmax of invertase of Aspergillus sp. was 15.15 U/mg protein, while the affinity for substrate shown by invertase was 1.25 mg/ml
Summary
Enzymes are biomolecules synthesized by the cells of an organism to allow biochemical reactions to occur under mild conditions and are known to catalyse many biochemical reactions (Li et al, 2018) They are generally considered to be very important in industrial processes (Roy and Prasad, 2017) due to the various advantages they have over inorganic catalysts. Invertase is usually preferred to glucose since they are sweeter and do not crystallize (Flores-Gallegos et al, 2012). Another enzyme related to these invertases are sucrases.
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