Abstract
To search for genes controlling high prolificacy of Chinese indigenous goats, differential display reverse transcription-polymerase chain reaction (DDRT-PCR) was used to screen differentially expressed cDNA bands in the sexually matured ovaries of 3-year-old prolific Jining Grey goats and monotocous Liaoning Cashmere goats with 24 combinations of three anchored primers and eight arbitrary primers. 22 expressed sequence tags (ESTs) were proved to be the positive bands by Northern hybridization. They comprised 10 known ESTs and 12 ESTs without homologous sequences in the GenBank. These results indicate that several genes such as GATA-4, metallothionein-like protein, CAT genes and unknown ESTs (CV983340 and CV983341) were expressed only in Jining Grey goats. Key words: Differential display reverse transcription-polymerase chain reaction, goat, ovary, prolificacy.
Highlights
The Jining Grey is a prolific indigenous goat breed, and Liaoning Cashmere is a monotocous indigenous goat breed in People’s Republic of China
To search for genes controlling high prolificacy of Chinese indigenous goats, differential display reverse transcription-polymerase chain reaction (DDRT-PCR) was used to screen differentially expressed cDNA bands in the sexually matured ovaries of 3-year-old prolific Jining Grey goats and monotocous Liaoning Cashmere goats with 24 combinations of three anchored primers and eight arbitrary primers. 22 expressed sequence tags (ESTs) were proved to be the positive bands by Northern hybridization. They comprised 10 known ESTs and 12 ESTs without homologous sequences in the GenBank. These results indicate that several genes such as GATA binding protein 4 (GATA-4), metallothionein-like protein, CAT genes and unknown ESTs (CV983340 and CV983341) were expressed only in Jining Grey goats
The two goat breeds have an obvious difference in litter size (2.94 and 1.18, respectively) (Feng et al, 2012; Tu, 1989), which made them possible models for elucidating the molecular mechanism of high prolificacy
Summary
The Jining Grey is a prolific indigenous goat breed, and Liaoning Cashmere is a monotocous indigenous goat breed in People’s Republic of China. The two goat breeds have an obvious difference in litter size (2.94 and 1.18, respectively) (Feng et al, 2012; Tu, 1989), which made them possible models for elucidating the molecular mechanism of high prolificacy. In the present study, we examined gene expression differences in the sexually matured ovaries of 3-year-old Jining Grey goats and Liaoning Cashmere goats. Differential display reverse transcription-polymerase chain reaction (DDRT-PCR), as a more powerful, sensitive and rapid method in detecting the differences in gene expression (Liang et al, 1994), was applied in the present study to search for genes or expressed sequence tags (ESTs) involved in high prolificacy of Chinese
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
