Abstract
The use of natural products with curative action is a common cultural practice in the Brazilian Amazon, but little explored scientifically. The effect of Hymenaea courbaril L sap extract and its contribution to wound healing in mice was evaluated. The antioxidant potential of the extract was studied using the radical 1,1-diphenyl-2-picrilhidrazil and 2,2'-azino-bis (3-ethyl benzothiazoline-6-sulfonic) acid radical, anion superoxide (O2-) radical tests, phenol and flavonoid content, proliferation assay, cell viability and migration. In vivo tests were performed on adult Swiss mice, submitted to back injury and treated with a formulation containing 2% sap extract, extracted with ethyl acetate. The animals were treated topically for 14 days, it was observed that the sap extract has antioxidant activity, inhibiting the production of reactive oxygen species, presented potential for the proliferation of fibroblasts and promoted cell migration. Morphometric analysis of wound closure on the 3rd day suggested that animals treated with Jatobá sap had a reduced area of injury when compared with the control group on the 3rd post-injury day for the treated group. On the 14th day, there was no difference between groups. The total closure of the wounds did not occur during the 14-day experiment, but through histological evaluation, it was found that there was re-epithelialization. The results suggest that Jatobá sap extract has the potential to induce the healing of skin wounds due to its antioxidant activity. Key words: Hymenaea courbaril L extract, cytotoxicity, antioxidant activity, wound.
Highlights
Wound healing is a complex cellular and biochemical process, necessary to restore structurally defective tissue and involves dynamic interactions between various types of cells, regardless of the causative agent (Grada et al, 2017)
The results presented in this work were produced to generate in vitro and in vivo knowledge on the antioxidant and healing activities of H. courbaril L (Jatobá) sap extract
Understanding the action mechanism of the reduction of diphenyl-2-picrylhydrazyl radical (DPPH) molecules and the main chemical classes present in H. courbaril L, it was suggested that the antioxidant action presented may be related to the presence of phenolic hydroxyl compounds available in the plant (Veggi et al, 2014) and to the presence of proanthocyanidin trimers and phenolic compounds such as taxifolin flavonoids identified in the chemical composition of the Jatobá sap extract
Summary
Wound healing is a complex cellular and biochemical process, necessary to restore structurally defective tissue and involves dynamic interactions between various types of cells, regardless of the causative agent (Grada et al, 2017). This process is directly related to the general conditions of the organism (De Prado et al, 2018). The medicinal plants were highlighted, an object of innumerous researches, which have become an effective alternative for treating many pathologies Such pathologies include skin wounds that affect the quality of life of patients and are considered one of the leading causes of physical disabilities (Oliveira et al, 2019). This tree occurs in all main types of low altitude tropical ecosystems and is well distributed throughout Brazil, occurring in almost all region, presenting uniform distribution in the Amazon (Silva et al, 2019)
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