Abstract
Inhibition of virulence genes expression of phytopathogenic bacteria can be done by degrading the acyl-homoserine lactone (AHL) compounds using AHL-lactonase. In this study, the AHL-lactonase genes were cloned and expressed from Bacillus cereus INT1c and Bacillus thuringiensis SGT3g in Escherichia coli, in order to understand the characteristics and biocontrol mechanisms of their AHL-lactonase. Amplification using aiiA primers succeeded to get 800 bp DNA fragments of AHL-latonase genes having a complete open reading frame (ORF) and encoding 250 amino acids. AHL-lactonase genes of B. cereus INT1c and B. thuringiensis SGT3g were expressed in E. coli BL21 (DE3) plasmid with T7 lysozyme coding sequence (pLysS). AHL-lactonase of the isolates is classified as metallo-β-lactamase superfamily domain and the AHL-lactonase inhibited violacein production of Chromobacterium violaceum. Key words: Acyl-homoserine lactone (AHL), lactonase, aiiA gene, cloning, Bacillus thuringiensis, Bacillus cereus.
Highlights
Quorum sensing is a communication mechanism between intracellular bacterial cells that depends on the density of cells and it has an important role in regulating gene expression such as the gene that is responsible for biofilm formation, virulence factors, antibiotics synthesis, sporulation, and bioluminescence (Galloway et al, 2011)
acyl-homoserine lactone (AHL)-lactonase genes of B. cereus INT1c and B. thuringiensis SGT3g were expressed in E. coli BL21 (DE3) plasmid with T7 lysozyme coding sequence
Insertion of AHL-lactonase genes in E. coli DH5α Bacterial isolates used in this study were detected having the ability to inhibit violacein production of C. violaceum
Summary
Quorum sensing is a communication mechanism between intracellular bacterial cells that depends on the density of cells and it has an important role in regulating gene expression such as the gene that is responsible for biofilm formation, virulence factors, antibiotics synthesis, sporulation, and bioluminescence (Galloway et al, 2011). Quorum sensing is mediated by an extracellular signaling molecule called autoinducer. Autoinducer molecules are detected and responded by their bacterial cells when the concentration in the environment is high. Bacteria produce autoinducers of various types and Gramnegative bacteria produce N-acyl homoserine lactone (AHL) autoinducer (Hentzer et al, 2002).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
