Abstract
In vitro propagation of Hoya wightii ssp. palniensis (Asclepiadaceae), a highly vulnerable and endemic plant species of Western Ghats, Tamil Nadu, India was carried out. Shoot tip explants were cultured on MS medium fortified with cytokinins (KN, BA, 2-iP and TDZ) in various concentrations and in combination with auxins (IBA, IAA and NAA). High frequency of shoot bud proliferation and multiplication was observed on KN (4.65 µM) + IBA (1.47 µM). Multiple shoot induction efficiency was increased on ascorbic acid (100 mg/l) supplemented medium along with KN (4.65 µM) +IBA (1.47 µM). Rhizogenesis was observed on MS medium supplemented with IBA (0.98 µM), plantlets produced through micropropagation were hardened with the survival success of 56%. The efficient plantlet regeneration protocol developed would aid ex situ conservation of this vulnerable species. Key words: Hoya wightii ssp. palniensis, in vitro propagation, Asclepiadaceae, plant growth regulators, ascorbic acid, micropropagation.
Highlights
Hoya is a genus of 300 species of tropical climbing plants, native to south Asia (India and China), Australia and Polynesia
Shoot tip explants were cultured on Murashige and Skoog (MS) medium fortified with cytokinins (KN, 6-benzyl adenine (BA), 2-iP and TDZ) in various concentrations and in combination with auxins (IBA, indole-3-acetic acid (IAA) and α- naphthalene acetic acid (NAA))
H. wightii ssp. palniensis has inherently poor seed setting nature and the only earlier instance of follicle known is on AS 1422 (SHC) of 1914 (Matthew, 1999b)
Summary
Hoya is a genus of 300 species of tropical climbing plants, native to south Asia (India and China), Australia and Polynesia. Palniensis, an endemic plant species, has a very restricted distribution only in the Western Ghats of Tamil Nadu, India. Mathew (1992) and Matthew (1996) reported the presence of H. wightii ssp. It is a trailing herb hanging from a rock in hills near river streams. There are 7 - 12 flowers in an umbel and each are 1.5 - 2 cm in diameter (Figure 1B). Fruit setting (Figure 1C) was observed by the authors under green house condition at Vattakanal conservation trust nursery, Kodaikanal during August 2007
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