Abstract
Molecular markers have proven to be powerful tools in research related with diversity, variability, and improvement of economically important tropical crops. This study analyzed eight physiological and morphological fruit characters of economic interest in the cultivated Mexican guava (Psidium guajava L.), and assessed the suitability of two sequence specific amplified polymorphism (SSAP) and simple sequence repeat (SSR) markers developed for their use in early selection of individual plants with given fruit characteristics. Principal component analysis (PCA) explained 79% of the morphological variability observed among accessions. S-SAP was more informative than AFLP for studies of variability and diversity in guava, the former marker showing higher percentage of polymorphism (90%) and more intraspecific variability (0.58). It was analyzed by cluster analysis using the unweighted pair group with arithmetic means (UPGMA) method the relationships between accessions from nine guava varieties. S-SAP dendrograms clustered varieties in better agreement with pulp color and fruit shape, suggesting a possible association of the S-SAP marker with quantitative trait loci (QTL) related to fruit physiological and morphological fruit characters. According to results, the microsatellites mPgCIR131, mPgCIR136 and mPgCIR161 might also be linked to QTL related to internal and external pulp thickness, pulp color, and soluble solids, indicating that the SSR markers developed are appropriate for their use in early selection of guava individuals having specific fruit features, therefore being suitable for molecular marker assisted selection (MAS) of the crop. Key words: Microsatellites, quantitative trait loci (QTL), sequence specific amplified polymorphism (SSAP), simple sequence repeat (SSR), Psidium guajava L.
Highlights
The guava (Psidium guajava L.) is a species of fruit tree belonging to the family Myrtaceae that is distributed in Campos-Rivero et al.tropical and subtropical regions, mostly in SoutheastAsia, Mexico, and Central and South America (Biffin et al, 2010)
Accessions grouped in five main groups in which pulp color showed not being determinant for discrimination of Principal component analysis (PCA) groups
PCA considering eight morphological characteristics of Mexican guava indicated that the two first components explain 79% of the morphological variability observed among the accessions
Summary
The guava (Psidium guajava L.) is a species of fruit tree belonging to the family Myrtaceae that is distributed in Campos-Rivero et al.tropical and subtropical regions, mostly in SoutheastAsia, Mexico, and Central and South America (Biffin et al, 2010). The guava (Psidium guajava L.) is a species of fruit tree belonging to the family Myrtaceae that is distributed in Campos-Rivero et al. The trees and fruits of the species are worldwide known for their ecological and economic relevance (Grattapaglia et al, 2012; Woodrow et al., 2012), and in many countries guava fruits are highly valued, in some of them even being a staple food (Liu and Yang, 2011). Some authors have proposed guava fruits are potentially nutraceutical due to their high contents of vitamins, minerals, and polyphenolic antioxidants (Hassimotto et al, 2005; Ho et al, 2012). Conventional breeding methods to improve woody species as guava are limited The wide variety of applications and ecological importance of the species there is constant progress of numerous research efforts for improving its agronomical characteristics.
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