Abstract
The expression of CD44 in osteoarthritis cartilage tissue and its effect in the generation and development of osteoarthritis (OA) were studied. Forty patients with primary OA who received joint arthroplasty were randomly included in this study as OA group. The normal articular cartilages as control group were obtained following Outerbridge standard from twenty patients who received amputation. The cartilages were fixed, decalcified, paraffin embedded and stained with hematoxylin and eosin (HE). Immunohistochemical staining method was used to determine CD44 expression. The fragments were divided into normal group, mild group, moderate group and severe group according to the severity of degeneration. CD44 expressed both in the control and OA group, and mainly localized in the cell membrane. The level of CD44 in OA group was significantly higher than that in the control group (P moderate group > severe group, and the three groups had significant differences (P<0.05). Pearson correlation analysis showed a significant negative correlation between the CD44 expression levels and the Mankin pathology score in the OA cartilage tissue (r = -6.013, P<0.01). Adhesion molecule CD44 highly expressed in osteoarthritis tissue, and gradually decreased with the severity of osteoarthritis lesions. Key words: Adhesion molecule CD44, osteoarthritis, cartilage tissue, immunohistochemical staining.
Highlights
Osteoarthritis (OA), known as osteoarthrosis, degenerative joint disease and senile arthritis, is a common chronic disabling disease
Histomorphology observation showed that in the control group, the surface of the cartilage tissue was slightly unsmooth with no crevice and a little bit of proliferation, and the cells were relatively structured
The results revealed that adhesion molecule CD44 expressed both in the control and OA groups and mainly concentrated at the cytomembrane (Figure 2)
Summary
Osteoarthritis (OA), known as osteoarthrosis, degenerative joint disease and senile arthritis, is a common chronic disabling disease. It has been demonstrated that CD44 plays an important role in local clearance of hyaluronan and mediates cell-matrix interactions involved in tumor cells metastasis, tumor formation and T cell extravasation (Liang et al, 2007). We did not find any report which showed its relationship with human articular cartilage cataplasia of OA. In our present work, the expression of CD44 in OA and different degree retrogression of articular cartilage was measured to reveal the correlation of CD44 with OA, and to further elucidate the mechanism of OA, providing theoretical basis for early diagnosis and treatment for OA. Average gray value was applied to analysze the expression differences of CD44 in the four groups and the correlation of CD44 expression with Mankin score. Probability values less than 0.05 were considered statistically significant (analysis was performed using SPSS for Windows, Version 16.0)
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