Abstract
The emergence of Escherichia coli isolates with multiple antibiotic resistant phenotypes is considered as a severe health concern. In the present work the antibacterial effect of following plants ( Herniaria hirsuta , Prunus avium , Rubia tinctorum and Sempervivum tectorum ) was examined. The bacterial model used for estimation of bacterial susceptibility is hospital multiple antibiotic resistant E. coli strain. E. coli ATCC 25922 was used for standard comparison of bacterial susceptibility. Leaves of H. hirsuta , R. tinctorum and S. tectorum as well as petioles of P. avium were collected. Ethanol and aqueous extract of each plant was prepared. Antibacterial activity was examined using the agar well diffusion method. Concentration of total phenols, flavonoids, tannins, antocyanins and saponins was determined in plant extracts. E. coli strain is resistant to four unrelated families of antibiotics. Antibacterial effect is proven for all examined plants. Ethanol extracts of H. hirsuta and P. avium have a more potent antibacterial effect than their aqueous extracts. Aqueous extracts of R. tinctorum and S. tectorum have higher antibacterial potential than theirs ethanol extracts. Examined plant extracts represent good candidates for more extensive research in view of their application in the treatment of multiple antibiotic resistant E.coli strains.
Highlights
Antibiotic resistance in pathogenic bacteria is a serious public health issue
R. tinctorum is a part of the Rubiaceae family and it is known as rich with antraquinone which is used as antiinflammatory, antimicrobial, antibacterial and antidiuretic drug (SWAIN, 1996)
Considering antraquinone from R. tinctorum, which is denoted as a main antibacterial compound, it is necessary to stress that antraquinone is poorly soluble in water as well as in ethanol on temperatures lower than boiling point (VOGEL, 2002)
Summary
Antibiotic resistance in pathogenic bacteria is a serious public health issue. E. coli can be responsible for enteric and diarrhoeal diseases, urinary tract infections, sepsis and meningitis (CHESNOKOVA et al 1999). Since 2003, E. coli strains producing extended spectrum β-lactamases (ESBLs), CTX-M-15 (cluster of cefotaxime resistant β-lactamases) have become increasingly common according to the Health Protection Agency. Isolates of strain A that possess an acquired AmpC βlactamase have recently been reported (WOODFORD et al 2007). Uropathogenic E. coli (UPEC) is the most common cause of community- and hospital-acquired urinary tract infections (UTIs). Isolates from uncomplicated community-acquired UTIs express a variety of virulence traits that promote the efficient colonization of the urinary tract. Uropathogenic E. coli is the primary causative agent for urinary tract infections (UTIs) and it has been presumed to be a predominantly extracellular
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