English

Abstract

By the method of microbiological synthesis were obtained and analyzed by electron impact mass-spectrometry 2H, 13C-labeled amino acids of a facultative methylotrophic bacterium Brevibacterium methylicum and an obligate methylotrophic bacterium Methylobacillus flagellatum and 2H, 13C-labeled amino acids of the total protein of biomass obtained on media containing as a source of stable isotopes [2H]methanol, [13C]methanol and 2H2O. It was also performed the incorporation of L-[2,3,4,5,6-2Н]phenylalanine, L-[3,5-2Н]tyrosine and L-[2,4,5,6,7-2Н]tryptopan into the membrane integral protein bacteriorhodopsin synthesised in purple membranes of photoorganotrophic halobacterium Halobacterium halobium ET 1001. For mass-spectrometric analysis the multicomponential mixtures of 2H- and 13C-labeled amino acids, derived from cultural media and protein hydrolysates after hydrolysis in 6 M 2HСl (3% phenol) and 2 M Ва(OH)2 were modified into N-benzyloxycarbonyl-derivatives of amino acids as well as into methyl esters of N-5(dimethylamino)naphthalene-1-sulfonyl chloride (dansyl) derivatives of [2H, 13С]amino acids,