Abstract
Uridine kinase (UK) and uracil phosphoribosyltransferase (UPRT) are enzymes in pyrimidine salvage pathway. UK or UPRT were reported as separate genes in bacteria. Amino acid sequence of OsUK/UPRT1 from rice shows homology to bacterial enzymes. Amino-terminal region is similar to UDK with an ATP/GTP-binding motif, whereas the carboxyl-terminal similar to UPP with two binding motifs for a uracil and a PRPP, respectively. Expression of OsUK/UPRT1 in an udk and/or upp mutant of Escherichia coli led to growth inhibition with 5-fluorouridine or 5-fluorouracil. These results suggest that the OsUK/UPRT1 product would be a natural fusion protein of two enzymes.
Highlights
The biosynthetic pathway of pyrimidines including uridine 5’-triphosphate (UTP) and cytidine 5’-triphosphate (CTP) is initiated by carbamoyl phosphate synthetase with glutamine and CO2
The sequence, OsUK/ UPRT1 cDNA, contained a full-length open reading frame consisted in 1850 bp and encoded a protein of approximately 55.2 kDa
Database analysis shows that OsUK/UPRT1 sequence is identical to a genomic region located in chromosome 9 in rice (Os09g0505800) and consisted of 14 exons
Summary
Faculty of Biotechnology, Jeju National University, Jeju, 690 - 756, Republic of Korea. Uridine kinase (UK) and uracil phosphoribosyltransferase (UPRT) are enzymes in pyrimidine salvage pathway. UK or UPRT were reported as separate genes in bacteria. Amino acid sequence of OsUK/UPRT1 from rice shows homology to bacterial enzymes. Amino-terminal region is similar to UDK with an ATP/GTP-binding motif, whereas the carboxyl-terminal similar to UPP with two binding motifs for a uracil and a PRPP, respectively. Expression of OsUK/UPRT1 in an udk and/or upp mutant of Escherichia coli led to growth inhibition with 5-fluorouridine or 5-fluorouracil. These results suggest that the OsUK/UPRT1 product would be a natural fusion protein of two enzymes
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