Abstract
Numerous cellulase producing mutant strains were obtained from the parent strain Trichoderma reesei Rut C-30 treated with 10 μg/mL ethidium bromide (EtBr) for 20 min following by 30 min-UV irradiation. During strain screening, size of hydrolysis zone and FPase activity were served as the indexes and mutant T. reesei EBUV-3 was finally selected. Nearly 1.4, 1.7, 2.0 and 1.3 folds enhancement of the activities of FPase, CMCase, β-glucosidase and xylanase were noted in T. reesei EBUV-3 compared with T. reesei Rut C-30. The Vmax of β-glucosidase and CMCase increased 1.7 and 1.4 folds. Meanwhile, the Km values were 2.6 and 1.5 folds lower than the parent strain. The saccharification of steam-exploded corn stover by T. reesei EBUV-3 increased 24%. After 48 h saccharification, the reducing sugars production of T. reesei EBUV-3 was determined to be 70% at an optimal condition of 55°C and pH 5.0. Our present experimental findings suggested that mutant T. reesei EBUV3 possesses a higher cellulase activity and stronger saccharifying ability compared with the parent strain. T. reesei EBUV-3 was promisingly potential to be a cellulase producer in application. © 2016 Friends Science Publishers
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