Abstract
Group A rotaviruses are characterized by large genomic diversity that is typically studied by genotyping of the VP7 glycoprotein, which defines G-type, and the protease-sensitive VP4, which defines P-type. The aim of this study was to examine the genotypes of group A rotavirus strains circulating in Senegal over a 7-year period between 2005 and 2011. ELISA positive stool specimens were analyzed by PAGE and were genotyped by multiplex hemi-nested RT-PCR. Data were analyzed with Epi Info 7 software. Rotavirus was found in 222 samples of stools of which 32 (14.41 %) were positive on PAGE. Five electropherotype patterns were identified, three long (L1, L2 or L3) and two short (S1 or S2). Twenty-three (23) samples had a long electropherotype and nine had a short electropherotype. One hundred strains (45.04%) were analyzed by VP7 genotyping, which identified five different genotypes: G1, G2, G3, G8 and G9. Ninety-three (93) samples (41.89%) were analyzed by VP4 genotyping which showed four different genotypes: P[4], P[6], P[8] and P[11] and mixed genotypes (1.8%). Seventy seven (34.68%) samples were genotyped for both VP7 and VP4. The G1P[8] strain was the most predominant strain followed by the G9P[8] strain. Rotavirus strains circulating in Senegal are genetically diverse. Genotypes G1P[8] and G9P[8] are the predominant strains. We also found mixed infections that could favor the emergence of new viral strains. Thus, regular monitoring of genotypes circulating in the country is required. Key words: Rotavirus, diarrhea, genotypes, children, Senegal.
Highlights
Mole ecular charac cteriza ation of group A ro otavirus ses c circula ating in n Senegal ov ver a 7--year p period (2005--2011)
Group A rotaviruses are e characteriz zed by large genomic div versity that is s typically sttudied by ge enotyping of the VP7 glycoprotein, which deffines G-type e, and the prrotease-sens sitive VP4, w which define es P-type
Detection rate and distribution of rotavirus according to year
Summary
Mole ecular charac cteriza ation of group A ro otavirus ses c circula ating in n Senegal ov ver a 7--year p period (2005--2011). The aim of this study was w to examiine the geno otypes of gro oup A rotavirrus strains c circulating in n Senegal over a 7-ye ear period be etween 2005. E positi ve stool spe ecimens werre analyzed by PAGE and were genotyped g by multiplex hemi-nested d RT-PCR. Five electropherrotype patterrns were ide entified, thre ee long (L1, L2 or L3) an nd two shortt (S1 or S2). Twentythree (23) samples s had d a long elec ctropherotyp pe and nine had a shortt electropherotype. One hundred strains (45.04%) were analyzed by VP7
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