English

Kumar Mishra Vinod,Singhyadav Punam,Arun Balasubramaniam,Kumar Vishwakarma Manish,Kumar Joshi Arun,Ch Ramesh,Kumar Gupta Pushpendra,Kumar Vasistha Neeraj

doi:10.5897/jpbcs2015.0514

Abstract

A wheat genotype named ‘PBW343+Gpc-B1+LR24’ containing Gpc-B1 gene linked with the DNA-based marker Xucw108,  developed at Choudhary Charan Singh University (CCSU), Meerut, through marker-assisted selection, was used as the donor parent to transfer the gene (Gpc-B1) for high grain protein content (GPC) into two popular cultivars (HUW234 and HUW468) of Eastern Gangetic Plains (EGP) of India. In both the cultivars, Gpc-B1 gene was introgressed through marker-assisted backcross breeding (MABB) which involved the following three steps: (i) Foreground selection; (ii) Screening for the carrier chromosome, and (iii) Recovery of recipient parent genome (RPG). Data on GPC (percent grain weight) was recorded for all selected individual plants from BC2F2:3 generation. The dominant marker Xucw108 was used for foreground selection, and heterozygous plants were identified through progeny testing. For RPG recovery, both genotypic and phenotypic selections were used. Introgression of high GPC gene into recipient background without yield loss was completed in 5 years, starting from F1 (2009-10) and completing it in BC2F5 (2013-2014). Ten selected single plants from BC2F3:4 had comparable yield with 26% higher GPC than the recurrent parent HUW 234. For the other parent HUW 468, eight selected plants had comparable yield with 34% higher GPC. Multi-row progenies (BC2F4 and BC2F5) of each selected plant were evaluated for yield traits with donor and recipient parents during 2012-2013 and 2013-2014. Two lines with significantly higher GPC were identified in each of the crosses with no yield penalty. The study reinforced the belief that marker assisted selection (MAS) in combination with phenotypic selection could be a useful strategy for the development of wheat genotypes with high GPC without sacrificing grain yield.   Key words: Triticum aestivum, grain protein content (GPC), marker assisted selection (MAS), carrier chromosome, recipient parent genome.

Highlights

Among cereals, wheat has highest grain protein content (GPC) ranging from 8 to 15% (Johnson et al, 1985) and is one of the most to 15% (Johnson et al, 1985) and is one of the most important components of nutritional value that directly affects bread making and pasta quality (Avni et al, 2014)
The experiment was conducted at the Agricultural Research Farm, Institute of Agricultural Sciences, Banaras Hindu University (BHU), Varanasi (25.15° N latitude; 82.59° E longitudes, 75.5 m above mean sea level, soil pH 7.4), which is located in the Eastern Gangetic Plains (EGP) of India
marker assisted selection (MAS) is becoming increasingly common in crop improvement programs (Collard and Mackill, 2008; Gupta et al, 2010), even in developing countries like India (Kumar et al, 2011; Vishwakarma et al, 2014) with greater integration of marker-assisted backcross breeding (MABB) approach in crop breeding programs around the world

Summary

Introduction

Wheat has highest GPC ranging from 8 to 15% (Johnson et al, 1985) and is one of the most to 15% (Johnson et al, 1985) and is one of the most important components of nutritional value that directly affects bread making and pasta quality (Avni et al, 2014). Since wheat is the most widely consumed food grain in the world, and provides staple food to 35% of the world population, occupying 17% of total arable land and 21.8% of the total area under food grain crops (Ortiz et al, 2008), breeding for nutritious wheat grain may be the most effective way of providing nutrition to human beings. The importance of this breeding objective is relevant to the thickly populated EGP of India, where 90% of the farmers are small land holders, who consume most of their farm produce as food.

Methods

Results

Conclusion