Abstract
In this study, a unique mucA mutation (designated mucA56) was introduced, which was characterized by deletion of bases 166-333, encoding MucA56 protein with the deletion of the trans-membrane region, which then was proved to be cytoplasmic with phoA-mucA fusion method. PAOmucA56 was constructed with homologous recombination; two PAO1 derivatives PAOmucA22 (PDO300) and PAOmucA56 displayed mucoid phenotype on pseudomonas isolation agar (PIA) agar, but PDO300 produced more alginate than PAOmucA56. Scanning confocal laser microscopy was used to observe the biofilm structures of the three strains during various biofilm development stages. PDO300 developed biofilm with low substratum coverage and high structural heterogeneity, while PAOmucA56 and PAO1 formed uniform biofilm with complete substratum coverage. The proteomes of crude protein extracts of biofilm cells revealed that there are 17 candidate proteins differentially expressed between the two kinds of biofilm, which were proteins involved in protein synthesis, MucA degradation, energy metabolism, carbon catabolism and amino acid metabolism and so on. We might conclude that alginate production may affect biofilm architecture, and proteins involved in protein synthesis, MucA degradation, energy metabolism, carbon catabolism and amino acid metabolism might play a role in biofilm development alternatively. Key words: Pseudomonas aeruginosa, mucA, alginate, biofilm, proteome.
Highlights
AP2/ethylene-response factors (ERF) transcription factors are characterized by a 60 to 70 amino acid DNA-binding domain binding to GCC box or GCC-like box in the ethylene-responsive genes (Jofuku et al, 1994; Ohme-Takagi and Shinshi, 1995)
The results suggest that HbEREBP2 may be involved in the regulation of jasmonate-mediated defense responses in laticifers of rubber tree
To elucidate the transcriptional regulation of the defense proteins, this study focused on the molecular characterization of a member of AP2/ERF transcription factors from laticifers of rubber tree
Summary
AP2/ERF transcription factors are characterized by a 60 to 70 amino acid DNA-binding domain binding to GCC box or GCC-like box in the ethylene-responsive genes (Jofuku et al, 1994; Ohme-Takagi and Shinshi, 1995). This domain is first identified in APETALA2 (AP2) from the Arabidopsis (Arabidopsis thaliana) and in the ethylene-responsive element binding proteins (EREBPs). The ERF family possesses only one AP2 domain and is further divided into two major subfamilies, the ERF subfamily and the CBF/DREB subfamily (Nakano et al, 2006). Members of AP2/ERF superfamily play important roles in regulating
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
