Abstract
Embracing micro-propagation method for large scale production of plantlets and also for protection of appropriate germplasm is a prerequisite that needs to be undertaken in order to develop a rapid in vitro regeneration protocol for Ocimum sanctum L. Shoot tips as well as nodal segments were subjected to numerous shoots inducement. Explants were cultured on Murashige and Skoog Basal Medium (MS) supplemented for different plants’ development controllers. HgCl 2 was utilized as a surface disinfecting agent. Nowadays, many researchers do not use HgCl 2 , so 1% sodium hypochlorite can be used. Cleaned explants were chiseled to 3-4cm length at right edges. The explants were inoculated vertically on the culture medium. The cultures were incubated at 25±2°C under cool fluorescent light. The photoperiod was set at 16 h light and 8 h darkness by automated timer. Data on shoot induction and expansion and root induction were recorded following three weeks of inoculation and utilized for figuring. Built up plantlets were transplanted in earthen pots under circumstances and outliving degree was recited. The practically viable surface sanitization medication for explants of O. sanctum was discovered at 0.1% HgCl 2 for 7 min. 1% sodium hypochlorite also showed same result. Maximum number of shoots per culture was recorded in MS medium containing 2.0 mg/l BAP in a mixture of 0.5 mg/l NAA. Regenerated shoots of O. sanctum were rooted most effectively in full MS medium supplemented with 1.0 mg/l NAA. It was observed that nodal segments are more responsive to micropropagation than shoot tips. This protocol is used to explore the opportunities of utilizing O. sanctum L., as important medicinal plant of Bangladesh, in modern medical health care system by rapid clonal propagation, and germplasm conservation. The developed plants were acclimatized in pot successfully and also maintained in normal environment. Keywords: Ocimum sanctum , micro-propagation, explants, nodal segments, medicinal plant, regeneration
Highlights
Bangladesh is a country which investigates sumptuously in medicamentary hereditary contrivance by virtue of its favorable agro-climatic condition and seasonal diversity
O. sanctum is not an endangered plant, but a valuable medicinal plant; so if we develop a method for in vitro regeneration of this plant, that can open many areas for further research and germplasm preservation
86% explants of O. sanctum were found free of contamination and healthy when treated with 0.1% HgCl2 for 7 min; they were the best
Summary
Bangladesh is a country which investigates sumptuously in medicamentary hereditary contrivance by virtue of its favorable agro-climatic condition and seasonal diversity. 80% rustic people depend on unregenerate explants (e.g. medicinal plants) for their chief healthcare (Rahman et al, 2013). When they are malignant medicinal plants, Bangladesh people trust on imported raw materials of pharmaceuticals. Within Ayurveda, tulsi is known as “The Incomparable One,” “Mother Medicine of Nature” and “The Queen of Herbs,” and is respected as an “elixir of life" that is without equivalent for both its restorative and profound properties (Singh et al, 2010).The hydrorefining of the aeronautical parts of O. sanctum is at the vegetative, botanical maturing stages. Concoction parts of the fundamental oils refined starting with three developmental phases of O. sanctum are ethyl isovalerate, α-pinene, sabinen, β-pinene, myrcene, 1,8-cineole, linalool, terpinen-4-ol, α-terpineol, estragol, eugenol, α-cisbergamotene, α-humlene, β-bisabolen, γ-elemene and methyl chavicol (Saharkhiz et al, 2015)
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