Abstract
Seeds of Helianthemum kahiricum have an excellent germination rate extending to about 90% within short time not more than four days after scarification (mechanical treatment) and a good protocol of disinfection. A high frequency of sprouting and shoot differentiation was observed in the primary cultures of nodal explants of H. kahiricum on Murashige and Skoog medium (MS) free growth regulators or with less concentration of kinetin (0.5 mg L -1 or 1.0 mg L -1 kin). In vitro proliferated shoot were multiplied rapidly by culture of shoot tips on MS with kinetin (0.5 mg L -1 ) which produced the greatest multiple shoot formation. The kinetin had a positive effect on the multiplication and growth, but a concentration that exceeded 2.0 mg.L -1 decreased the growth. A high frequency of rooting with development of healthy roots was observed from shoots cultured on MS/8 medium hormone-free. Key words : Helianthemum kahiricum, in vitro germination, multiplication, axillary buds. Abbreviation: Zea, Zeatin (cytokinine); IAA, indole-3-acetic acid (auxin); IBA, indole-3-butyric acid (auxin); Kin, kinetin (cytokinine); MS, Murashige and Skoog medium; NAA, 2-naphthalene acetic acid (auxin); 2iP, 2 isopentenyladenine (cytokinine).
Highlights
Helianthemum kahiricum (H. kahiricum) is a perennial herb widely found around the Mediterranean basin (Raynaud, 1987)
Whatever the amount of kin in the medium, we find that indole-3-butyric acid (IBA) promoted rooting with a rate of 16% and the NAA with a rate of
H. kahiricum is considered threatened in an extremely precarious situation. Micropropagation of this plant was an attempt conducted from nodal explants of plantlets in vitro to preserve/conserve this species by the micropropagation technique which aims to safeguard biodiversity; thereby developing an appropriate protocol for disinfecting seeds which was necessary to achieve aseptic cultures
Summary
Helianthemum kahiricum (called R'guiga or Chaal in Tunisia) (H. kahiricum) is a perennial herb widely found around the Mediterranean basin (Raynaud, 1987). H. kahiricum is an appressed, grey-canescent perennial low shrub that reaches up to 10-25 cm long with many, branched stems. The fruit is an ovoid-globose and hairy capsule with ovoidcompressed, smooth, and brownish. This is a chaméphyte (Escudero et al, 2007), family of Cistaceae, arid regions and semi-arid areas (Perez- Garcia and Gonzalez- Benito, 2006). Despite its ecological and economic interests, this plant is a rare endemic flora of the western basin of the Mediterranean (Escudero et al., 2007), as a result of overgrazing (Aidoud et al, 2006)
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