Abstract
Forty-three endophytic fungal isolates were separated from the healthy twigs and barks of the hybrid poplar 'Neva' of Populus deltoides Marsh × Populus nigra L. Eleven distinct isolates were selected for further taxonomical identification by morphological traits and internal transcribed spacer (ITS) rRNA gene sequence analysis. Eight genera namely Alternaria, Aureobasidium, Botryosphaeria, Hyalodendriella, Mycosphaerella, Peyronellaea, Phoma and Valsa were identified on the basis of their morphological characterizations. Of them, the most frequent genus was Alternaria (that is, Ponipodef01, Ponipodef04 and Ponipodef07). Their ITS-rDNA sequences were compared with those available in the GenBank database to obtain the closest related species by BLAST analysis. A modified thin layer chromatography-bioautography assay was used to detect the antimicrobial activity of the n-butanol extracts of mycelia and culture filtrates of the isolates. Most of the fungal isolates were observed to have antibacterial activity, and antibacterial components mainly existed in mycelia. Two isolates Ponipodef05 and Ponipodef12 were also observed to have antifungal activity. The endophytic fungi isolated from the hybrid poplar 'Neva' of P. deltoides Marsh × P. nigra L. may be used as potential producers of antimicrobial natural products. Key words: Poplar hybrid 'Neva', endophytic fungi, extract, antimicrobial activity.
Highlights
Plant endophytic fungi are defined as the fungi which spend the whole or part of their lifecycle colonizing inside the healthy tissues of the host plant, typically causing no apparent symptoms of disease (Wilson, 1995; Zhang et al, 2006; Rodriguez et al, 2009)
The twigs and barks were eliminated epidermis and cut into the segments (5 × 5 mm), which were placed in a 90 mm diameter Petri-dish containing potato dextrose agar (PDA) medium supplemented with streptomycin sulfate (500 mg/L) to suppress bacteria growth
The colonization frequency (CF) of each endophyte was calculated according to the method of Hata and Futai (1995), where CF = (NCOL/Nt) × 100 where NCOL is the number of segments colonized by each fungus and Nt is the total number of segments
Summary
Plant endophytic fungi are defined as the fungi which spend the whole or part of their lifecycle colonizing inside the healthy tissues of the host plant, typically causing no apparent symptoms of disease (Wilson, 1995; Zhang et al, 2006; Rodriguez et al, 2009). Many metabolites produced by the endophytes have been tested to Species and hybrids of Populus (Salicaceae) are of worldwide importance in the production of fibre and energy (Nixon et al, 2001). The poplar cultivar 'Neva', which is the hybrid of Populus deltoides Marsh × Populus nigra L., is one of the most important salicaceous woody plants in subtropical and temperate regions with its desirable characteristics such as drought, insect and disease resistances (Fang et al, 2007; Zhou et al, 2008). To the best of our knowledge, there is no reported study on the endophytic fungi associated with the poplar hybrid Zhong et al 3925. The objective of this study was to isolate and identify the endophytic fungi from the poplar hybrid 'Neva' as well as to examine their antimicrobial activities
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
