Abstract
The aim of this study was to investigate the anthelmintic effect of pumpkin seeds (Cucurbita maxima) against Aspiculuris tetraptera in naturally infected mice. For this purpose, the natural infection was determined by the cellophane tape (on the anal region), and the centrifugal flotation methods of stool samples in approximately 150 male Swiss albino mice (27 to 35 g). The infected mice (29 animals) were divided into four groups. The animals in the first (G1) and second groups (G2) received water and ethanol extracts of pumpkin seeds orally at the dose suggested for human for 7 days, respectively. The mice in the positive control group (G3) were treated with ivermectin intramuscularly at a dose of 0.2 mg/kg body weight. The mice in fourth groups (G4) received the same amount of serum physiologic orally. The mice were housed in clean polypropylene cages and maintained under standard laboratory conditions at an ambient temperature of 20±2°C with 45% relative humidity and a 12 h light dark cycle. At the eighth day of the study, all animals were killed humanly following inhalation anaesthesia. After euthanasia, the number of parasites in the intestine was counted. Data obtained from the treatment groups were compared using one-way ANOVA. The percentage efficacy of the drugs was calculated. The results of the study showed that the efficacies for water (G1) or ethanol (G2) extracts of pumpkin seed and ivermectin were 81, 85 and 91% compared with the negative control, respectively. These results revealed that pumpkin seed has high anthelmintic activity against nematodes as well as its continued use in traditional medicine for the treatment of helminthiasis. Key words: Aspiculuris tetraptera, mice, anthelmintic effect, efficacy, Cucurbita maxima.
Highlights
The traditional medicinal plant use in all cultures is well documented
The purpose of this study was to evaluate the anthelmintic efficacy of pumpkin seeds (Cucurbita maxima) in mice naturally infected with Aspiculuris tetraptera
The efficacies for ivermectin (G3), water (G1) and ethanol (G2) extracts were 91, 85 and 81% compared to the negative control group (G4), respectively
Summary
The traditional medicinal plant use in all cultures is well documented. Plants have been the main component ofJ. According to the data of the World Health Organisation, 80% of people use these treatment methods in developing countries and 3.3 billion people benefit from medical plants as treatment. In recent years, their usage in developed countries has increased dramatically (Baytop et al, 1999; Saraçoğlu et al, 2005; Mindell, 2003; Saraç, 2006). Their usage in developed countries has increased dramatically (Baytop et al, 1999; Saraçoğlu et al, 2005; Mindell, 2003; Saraç, 2006) The medicinal plants such as garlic, black cumin, stinger and pumpkin seeds have been used extensively in the treatments of various maladies as traditional medicine (Beech et al, 2011)
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